Kunjin virus replicon vaccine vectors induce protective CD8(+) T-cell immunity

Anraku, Itaru, Harvey, Tracey J., Linedale, Richard, Gardner, Joy, Harrich, David, Suhrbier, Andreas and Khromykh, Alexander A. (2002) Kunjin virus replicon vaccine vectors induce protective CD8(+) T-cell immunity. Journal of Virology, 76 8: 3791-3799. doi:10.1128/JVI.76.8.3791-3799.2002

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Author Anraku, Itaru
Harvey, Tracey J.
Linedale, Richard
Gardner, Joy
Harrich, David
Suhrbier, Andreas
Khromykh, Alexander A.
Title Kunjin virus replicon vaccine vectors induce protective CD8(+) T-cell immunity
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
Publication date 2002-04
Year available 2002
Sub-type Article (original research)
DOI 10.1128/JVI.76.8.3791-3799.2002
Open Access Status File (Publisher version)
Volume 76
Issue 8
Start page 3791
End page 3799
Total pages 9
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Subject 730213 Preventive medicine
Abstract The ability of self-replicating RNA (replicon) vaccine vectors derived from the Australian flavivirus Kunjin (KUN) to induce protective alphabeta CD8(+) T-cell responses was examined. KUN replicons encoding a model immunogen were delivered by three different vaccine modalities: (i) as naked RNA transcribed in vitro, (ii) as plasmid DNA constructed to allow in vivo transcription of replicon RNA by cellular RNA polymerase II (DNA based), and (iii) as replicon RNA encapsidated into virus-like particles. A single immunization with any of these KUN replicon vaccines induced CD8(+) T-cell responses at levels comparable to those induced by recombinant vaccinia virus encoding the same immunogen. Immunization with only 0.1 mug of DNA-based KUN replicons elicited CD8(+) T-cell responses similar to those seen after immunization with 100 mug of a conventional DNA vaccine. Naked RNA immunization with KUN replicons also protected mice against challenges with recombinant vaccinia virus and B16 tumor cells. These results demonstrate the value of KUN replicon vectors for inducing protective antiviral and anticancer CD8(+) T-cell responses.
Keyword Virology
Equine Encephalitis-virus
Simian Immunodeficiency Virus
Genes In-vitro
Heterologous Genes
Langerhans Cells
Expression Vectors
Flavivirus Kunjin
Dendritic Cells
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 56 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 04 May 2006, 19:17:16 EST