Rumen cellulosomics: divergent fiber-degrading strategies revealed by comparative genome-wide analysis of six ruminococcal strains

Dassa, Bareket, Borovok, Ilya, Ruimy-Israeli, Vered, Lamed, Raphael, Flint, Harry J., Duncan, Sylvia H., Henrissat, Bernard, Coutinho, Pedro, Morrison, Mark, Mosoni, Pascale, Yeoman, Carl J., White, Bryan A. and Bayer, Edward A. (2014) Rumen cellulosomics: divergent fiber-degrading strategies revealed by comparative genome-wide analysis of six ruminococcal strains. PLoS One, 9 7: e99221.1-e99221.14. doi:10.1371/journal.pone.0099221


Author Dassa, Bareket
Borovok, Ilya
Ruimy-Israeli, Vered
Lamed, Raphael
Flint, Harry J.
Duncan, Sylvia H.
Henrissat, Bernard
Coutinho, Pedro
Morrison, Mark
Mosoni, Pascale
Yeoman, Carl J.
White, Bryan A.
Bayer, Edward A.
Title Rumen cellulosomics: divergent fiber-degrading strategies revealed by comparative genome-wide analysis of six ruminococcal strains
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-07-03
Sub-type Article (original research)
DOI 10.1371/journal.pone.0099221
Open Access Status DOI
Volume 9
Issue 7
Start page e99221.1
End page e99221.14
Total pages 14
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2015
Language eng
Formatted abstract
Background: A complex community of microorganisms is responsible for efficient plant cell wall digestion by many herbivores, notably the ruminants. Understanding the different fibrolytic mechanisms utilized by these bacteria has been of great interest in agricultural and technological fields, reinforced more recently by current efforts to convert cellulosic biomass to biofuels.

Methodology/Principal Findings: Here, we have used a bioinformatics-based approach to explore the cellulosome-related components of six genomes from two of the primary fiber-degrading bacteria in the rumen: Ruminococcus flavefaciens (strains FD-1, 007c and 17) and Ruminococcus albus (strains 7, 8 and SY3). The genomes of two of these strains are reported for the first time herein. The data reveal that the three R. flavefaciens strains encode for an elaborate reservoir of cohesin- and dockerin-containing proteins, whereas the three R. albus strains are cohesin-deficient and encode mainly dockerins and a unique family of cell-anchoring carbohydrate-binding modules (family 37).

Conclusions/ Significance: Our comparative genome-wide analysis pinpoints rare and novel strain-specific protein architectures and provides an exhaustive profile of their numerous lignocellulose-degrading enzymes. This work provides blueprints of the divergent cellulolytic systems in these two prominent fibrolytic rumen bacterial species, each of which reflects a distinct mechanistic model for efficient degradation of cellulosic biomass. 
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
UQ Diamantina Institute Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 14 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 14 times in Scopus Article | Citations
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