Vegfd can compensate for loss of Vegfc in zebrafish facial lymphatic sprouting

Astin, Jonathan W., Haggerty, Michael J. L., Okuda, Kazuhide S., Le Guen, Ludovic, Misa, June P., Tromp, Alisha, Hogan, Benjamin M., Crosier, Kathryn E. and Crosier, Philip S. (2014) Vegfd can compensate for loss of Vegfc in zebrafish facial lymphatic sprouting. Development (Cambridge), 141 13: 2680-2690. doi:10.1242/dev.106591

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Author Astin, Jonathan W.
Haggerty, Michael J. L.
Okuda, Kazuhide S.
Le Guen, Ludovic
Misa, June P.
Tromp, Alisha
Hogan, Benjamin M.
Crosier, Kathryn E.
Crosier, Philip S.
Title Vegfd can compensate for loss of Vegfc in zebrafish facial lymphatic sprouting
Journal name Development (Cambridge)   Check publisher's open access policy
ISSN 1477-9129
0950-1991
Publication date 2014-07
Year available 2014
Sub-type Article (original research)
DOI 10.1242/dev.106591
Open Access Status File (Publisher version)
Volume 141
Issue 13
Start page 2680
End page 2690
Total pages 11
Place of publication Cambridge, United Kingdom
Publisher The Company of Biologists
Collection year 2015
Language eng
Abstract Lymphangiogenesis is a dynamic process that involves the sprouting of lymphatic endothelial cells (LECs) from veins to form lymphatic vessels. Vegfr3 signalling, through its ligand Vegfc and the extracellular protein Ccbe1, is essential for the sprouting of LECs to form the trunk lymphatic network. In this study we determined whether Vegfr3, Vegfc and Ccbe1 are also required for development of the facial and intestinal lymphatic networks in the zebrafish embryo. Whereas Vegfr3 and Ccbe1 are required for the development of all lymphatic vessels, Vegfc is dispensable for facial lymphatic sprouting but not for the complete development of the facial lymphatic network. We show that zebrafish vegfd is expressed in the head, genetically interacts with ccbe1 and can rescue the lymphatic defects observed following the loss of vegfc. Finally, whereas knockdown of vegfd has no phenotype, double knockdown of both vegfc and vegfd is required to prevent facial lymphatic sprouting, suggesting that Vegfc is not essential for all lymphatic sprouting and that Vegfd can compensate for loss of Vegfc during lymphatic development in the zebrafish head.
Keyword Vegfd
Lymphatic
Vegfc
Zebrafish
Lymphangiogenesis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 11 times in Scopus Article | Citations
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