Prostaglandin E and F receptor expression and myometrial sensitivity at labor onset in the sheep

Palliser H.K., Hirst J.J., Ooi G.T., Rice G.E., Dellios N.L., Escalona R.M., Parkington H.C. and Young I.R. (2005) Prostaglandin E and F receptor expression and myometrial sensitivity at labor onset in the sheep. Biology of Reproduction, 72 4: 937-943. doi:10.1095/biolreprod.104.035311


Author Palliser H.K.
Hirst J.J.
Ooi G.T.
Rice G.E.
Dellios N.L.
Escalona R.M.
Parkington H.C.
Young I.R.
Title Prostaglandin E and F receptor expression and myometrial sensitivity at labor onset in the sheep
Journal name Biology of Reproduction   Check publisher's open access policy
ISSN 0006-3363
Publication date 2005
Sub-type Article (original research)
DOI 10.1095/biolreprod.104.035311
Volume 72
Issue 4
Start page 937
End page 943
Total pages 7
Language eng
Subject 1307 Cell Biology
1309 Developmental Biology
2710 Embryology
Abstract Prostaglandins (PGs) play a pivotal role in the initiation and progression of term and preterm labor. Uterine activity is stimulated primarily by PGE 2 and PGF2α acting on prostaglandin E (EP) and prostaglandin F (FP) receptors, respectively. Activation of FP receptors strongly stimulates the myometrium, whereas stimulation of EP receptors may lead to contraction or relaxation, depending on the EP subtype (EP1-4) expression. Thus, the relative expression of FP and EP1-4 may determine the responsiveness to PGE2 and PGF2α. The aims of this study were to characterize the expression of EP1-4 and FP in intrauterine tissues and placentome, together with myometrial responsiveness to PG, following the onset of dexamethasone-induced preterm and spontaneous term labor. Receptor mRNA expression was measured using quantitative real-time polymerase chain reaction using species-specific primers. There was no increase in myometrial contractile receptor expression at labor onset, nor was there a change in sensitivity to PGE2 and PCF2α. This suggests expression of these receptors reaches maximal levels by late gestation in sheep. Placental tissue showed a marked increase in EP2 and EP3 receptor expression, the functions of which are unknown at this time. Consistent with previous reports, these results suggest that PG synthesis is the main factor in the regulation of uterine contractility at labor. This is the first study to simultaneously report PG E and F receptor expression in the key gestational tissues of the sheep using species-specific primers at induced-preterm and spontaneous labor onset.
Keyword Parturition
Placenta
Pregnancy
Uterus
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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