Production of Entomopathogenic Viruses

Reid, Steve, Chan, Leslie and van Oers, Monique M. (2014). Production of Entomopathogenic Viruses. In Juan A. Morales-Ramos, M. Guadalupe Rojas and David I. Shapiro-Ilan (Ed.), Mass Production of Beneficial Organisms: Invertebrates and Entomopathogens (pp. 437-482) London: Elseiver. doi:10.1016/B978-0-12-391453-8.00013-3

Author Reid, Steve
Chan, Leslie
van Oers, Monique M.
Title of chapter Production of Entomopathogenic Viruses
Title of book Mass Production of Beneficial Organisms: Invertebrates and Entomopathogens
Place of Publication London
Publisher Elseiver
Publication Year 2014
Sub-type Research book chapter (original research)
DOI 10.1016/B978-0-12-391453-8.00013-3
Open Access Status
Year available 2014
ISBN 9780123914156
Editor Juan A. Morales-Ramos
M. Guadalupe Rojas
David I. Shapiro-Ilan
Chapter number 13
Start page 437
End page 482
Total pages 46
Total chapters 20
Collection year 2015
Language eng
Formatted Abstract/Summary
Of all the entomopathogenic viruses, baculoviruses show the most potential to control lepidopteran pests on crops. Many baculoviruses produced in vivo have found commercial success on a range of crops. However, wider use of these highly specific, safe, and environmentally sustainable biopesticides is likely if they can be produced in vitro using readily scalable cell culture processes. In this chapter, the status of knowledge related to baculovirus infections of cells in culture is reviewed in relation to the cell lines used, the media required to grow the cells, and the genetic stability and yield of the viruses following in vitro infections. Helicoverpa armigera nucleopolyhedrovirus is used as a case study to indicate that at current yields an in vitro production process is feasible at the 10,000 l bioreactor scale; however, further work is required to resolve quality control issues in production. The application of systems biology to improve yields further and make commercialization more attractive is also addressed.
Keyword Baculoviruses
Culture media
Genome instability
In Vitro Production
Quality control
Systems biology
Q-Index Code B1
Q-Index Status Confirmed Code
Institutional Status UQ

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Created: Fri, 04 Jul 2014, 10:04:47 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences