Redefinition of the human mast cell transcriptome by deep-CAGE sequencing

Motakis, Efthymios, Guhl, Sven, Ishizu, Yuri, Itoh, Masayoshi, Kawaji, Hideya, de Hoon, Michiel, Lassman, Timo, Carninci, Piero, Hayashizaki, Yoshihide, Zuberbier, Torsten, Forrest, Alistair R. R., Babina, Magda, for The FANTOM Consortium, Wells, Christine, Wolvetang, Ernst, Beckhouse, Anthony, Fearnley, Liam, Hitchens, Kelly, Kenna, Tony, Le Cao, Kim-Anh, Mason, Elizabeth, Nielsen, Lars, Vijayan, Dipti, Blumenthal, Antje, Ovchinnikov, Dmitry and Briggs, James (2014) Redefinition of the human mast cell transcriptome by deep-CAGE sequencing. Blood, 123 17: 58-67. doi:10.1182/blood-2013-02-483792


Author Motakis, Efthymios
Guhl, Sven
Ishizu, Yuri
Itoh, Masayoshi
Kawaji, Hideya
de Hoon, Michiel
Lassman, Timo
Carninci, Piero
Hayashizaki, Yoshihide
Zuberbier, Torsten
Forrest, Alistair R. R.
Babina, Magda
for The FANTOM Consortium
Wells, Christine
Wolvetang, Ernst
Beckhouse, Anthony
Fearnley, Liam
Hitchens, Kelly
Kenna, Tony
Le Cao, Kim-Anh
Mason, Elizabeth
Nielsen, Lars
Vijayan, Dipti
Blumenthal, Antje
Ovchinnikov, Dmitry
Briggs, James
Title Redefinition of the human mast cell transcriptome by deep-CAGE sequencing
Journal name Blood   Check publisher's open access policy
ISSN 0006-4971
1528-0020
Publication date 2014-04-24
Sub-type Article (original research)
DOI 10.1182/blood-2013-02-483792
Volume 123
Issue 17
Start page 58
End page 67
Total pages 10
Place of publication Washington, DC, United States
Publisher American Society of Hematology
Collection year 2015
Language eng
Formatted abstract
Key Points
• Generated a reference transcriptome for ex vivo, cultured, and stimulated mast cells, contrasted against a broad collection of primary cells.
• Identified BMPs as function-modulating factors for mast cells.

Mast cells (MCs) mature exclusively in peripheral tissues, hampering research into their developmental and functional programs. Here, we employed deep cap analysis of gene expression on skin-derived MCs to generate the most comprehensive view of the human MC transcriptome ever reported. An advantage is that MCs were embedded in the FANTOM5 project, giving the opportunity to contrast their molecular signature against a multitude of human samples. We demonstrate that MCs possess a unique and surprising transcriptional landscape, combining hematopoietic genes with those exclusively active in MCs and genes not previously reported as expressed by MCs (several of them markers of unrelated tissues). We also found functional bone morphogenetic protein receptors transducing activatory signals in MCs. Conversely, several immune-related genes frequently studied in MCs were not expressed or were weakly expressed. Comparing MCs ex vivo with cultured counterparts revealed profound changes in the MC transcriptome in in vitro surroundings. We also determined the promoter usage of MC-expressed genes and identified associated motifs active in the lineage. Befitting their uniqueness, MCs had no close relative in the hematopoietic network (also only distantly related with basophils). This rich data set reveals that our knowledge of human MCs is still limited, but with this resource, novel functional programs of MCs may soon be discovered.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Published FANTOM authors are "for the FANTOM consortium".

 
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Created: Thu, 26 Jun 2014, 14:29:55 EST by Cathy Fouhy on behalf of Institute for Molecular Bioscience