Can proinflammatory cytokine gene expression explain multifidus muscle fiber changes after an intervertebral disc lesion?

Hodges, Paul W., James, Gregory, Blomster, Linda, Hall, Leanne, Schmid, Annina B., Shu, Cindy, Little, Chris and Melrose, James (2014) Can proinflammatory cytokine gene expression explain multifidus muscle fiber changes after an intervertebral disc lesion?. Spine, 39 13: 1010-1017. doi:10.1097/BRS.0000000000000318

Author Hodges, Paul W.
James, Gregory
Blomster, Linda
Hall, Leanne
Schmid, Annina B.
Shu, Cindy
Little, Chris
Melrose, James
Title Can proinflammatory cytokine gene expression explain multifidus muscle fiber changes after an intervertebral disc lesion?
Journal name Spine   Check publisher's open access policy
ISSN 1528-1159
Publication date 2014-06-01
Year available 2014
Sub-type Article (original research)
DOI 10.1097/BRS.0000000000000318
Open Access Status
Volume 39
Issue 13
Start page 1010
End page 1017
Total pages 8
Place of publication Philadelphia, United States
Publisher Lippincott Williams and Wilkins
Collection year 2015
Language eng
Formatted abstract
Study Design. Longitudinal case-controlled animal study.

Objective. To investigate the effect of an intervertebral disc (IVD) lesion on the proportion of slow, fast, and intermediate muscle fiber types in the multifidus muscle in sheep, and whether muscle fiber changes were paralleled by local gene expression of the proinflammatory cytokines tumor necrosis factor [alpha] (TNF-[alpha]) and interleukin 1-[beta].

Summary of Background Data. Structure and behavior of the multifidus muscle change in acute and chronic back pain, but the mechanisms are surprisingly poorly understood and the link between structure and behavior is tenuous. Although changes in muscle fiber types have the potential to unify the observations, the effect of injury on muscle fiber distribution has not been adequately tested, and understanding of possible mechanisms is limited.

Methods. The L1–L2, L3–L4, and L5–L6 IVDs of 11 castrated male sheep received anterolateral lesions. Six control sheep underwent no surgical procedures. Multifidus muscle tissue was harvested at L4 for muscle fiber analysis using immunohistochemistry and L2 for cytokine analysis with polymerase chain reaction for local gene expression of TNF-[alpha] and interleukin-1[beta].

Results. The proportion of slow muscle fibers in multifidus was significantly less in the lesioned animals both ipsilateral and contralateral to the IVD lesion. The greatest reduction in slow fibers was in the deep medial muscle region. A greater prevalence of intermediate fibers on the uninjured side implies a delayed fiber-type transformation on that side. TNF-[alpha] gene expression in multifidus was greater on both sides in the lesion animals than in the muscle of control animals. Interleukin-1[beta] was increased only on the injured side.

Conclusion. These data provide evidence of muscle fiber changes after induction of an IVD lesion and a parallel increase in TNF-[alpha] expression. Proinflammatory cytokine changes provide a novel mechanism to explain behavioral and structural changes in multifidus.

Level of Evidence: N/A
Keyword Multifidus muscle
Tumor Necrosis Factor Alpha
Intervertebral disc
Slow muscle fibers
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2015 Collection
School of Health and Rehabilitation Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
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