EST sequencing of Meloidogyne javanica infected pineapple root tissues reveals changes in gene expression during root-knot nematode induced gall formation

Moyle, Richard L. and Botella, Jose R. (2014) EST sequencing of Meloidogyne javanica infected pineapple root tissues reveals changes in gene expression during root-knot nematode induced gall formation. Tropical Plant Biology, 7 2: 43-52. doi:10.1007/s12042-014-9136-6


Author Moyle, Richard L.
Botella, Jose R.
Title EST sequencing of Meloidogyne javanica infected pineapple root tissues reveals changes in gene expression during root-knot nematode induced gall formation
Formatted title
EST sequencing of Meloidogyne javanica infected pineapple root tissues reveals changes in gene expression during root-knot nematode induced gall formation
Journal name Tropical Plant Biology   Check publisher's open access policy
ISSN 1935-9756
1935-9764
Publication date 2014-06
Sub-type Article (original research)
DOI 10.1007/s12042-014-9136-6
Open Access Status
Volume 7
Issue 2
Start page 43
End page 52
Total pages 10
Place of publication New York, NY, United States
Publisher Springer
Collection year 2015
Language eng
Formatted abstract
An expressed sequence tag project has been performed to survey a range of expressed sequences in the vascular cylinder tissue of pineapple roots infected with the root-knot nematode Meloidogyne javanica. A total of 4,102 EST sequences were obtained, comprising of 1,298 early infection clones, 2,461 late infection clones and 343 non-infected root tip clones. Clone redundancy was 34.4 %, with the 4,102 EST sequences clustering into 2,976 contigs comprising of 286 clusters and 2,690 singletons. A comparison of the most abundant clones isolated from the early and late infection libraries revealed significant differences in the transcriptomes of the vascular cylinder at early and late infection stages. Northern analysis and quantitative real time PCR confirmed a variety of genes including a metallothinein-like protein, an alpha tubulin, a phosphoglyceratemutase, a glyceradehyde phosphate dehydrogenase, a mannose-binding lectin and four previously undiscovered sequences are differentially expressed during gall formation. Analysis of clone distribution by functional classification revealed that the late infection library contains a higher proportion of clones associated with oxidative stress responses and the detoxification of free radicals. The EST and contig sequence collection, bioinformatic data and functional classification information is available through an online pineapple database resource housed at http://genet.imb.uq.edu.au/Pineapple/index.html.
Keyword Ananas comosus
Enolase
M. javanica
Nematode infection
Xyloglucan endotransglycosylase
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2015 Collection
 
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Created: Mon, 23 Jun 2014, 16:58:01 EST by Dr Richard Moyle on behalf of School of Agriculture and Food Sciences