Transcriptomic analyses reveal the adaptive features and biological differences of guts from two invasive whitefly species

Ye, Xiao-Dong, Su, Yun-Lin, Zhao, Qiong-Yi, Xia, Wen-Qiang, Liu, Shu-Sheng and Wang, Xiao-We (2014) Transcriptomic analyses reveal the adaptive features and biological differences of guts from two invasive whitefly species. BMC Genomics, 15 1: . doi:10.1186/1471-2164-15-370


Author Ye, Xiao-Dong
Su, Yun-Lin
Zhao, Qiong-Yi
Xia, Wen-Qiang
Liu, Shu-Sheng
Wang, Xiao-We
Title Transcriptomic analyses reveal the adaptive features and biological differences of guts from two invasive whitefly species
Journal name BMC Genomics   Check publisher's open access policy
ISSN 1471-2164
Publication date 2014-05-15
Year available 2014
Sub-type Article (original research)
DOI 10.1186/1471-2164-15-370
Open Access Status DOI
Volume 15
Issue 1
Total pages 12
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2015
Language eng
Abstract Background: The gut of phloem feeding insects is critical for nutrition uptake and xenobiotics degradation. However, partly due to its tiny size, genomic information for the gut of phloem feeding insects is limited.Results: In this study, the gut transcriptomes of two species of invasive whiteflies in the Bemisia tabaci complex, Middle East Asia Minor 1 (MEAM1) and Mediterranean (MED), were analyzed using the Illumina sequencing. A total of 12,879 MEAM1 transcripts and 11,246 MED transcripts were annotated with a significant Blastx hit. In addition, 7,000 and 5,771 gut specific genes were respectively identified for MEAM1 and MED. Functional analyses on these gut specific genes demonstrated the important roles of gut in metabolism of insecticides and secondary plant chemicals. To reveal the molecular difference between guts of MEAM1 and MED, a comparison between gut transcriptomes of the two species was conducted and 3,910 pairs of orthologous genes were identified. Based on the ratio of nonsynonymous and synonymous substitutions, 15 genes were found evolving under positive selection. Many of those genes are predicted to be involved in metabolism and insecticide resistance. Furthermore, many genes related to detoxification were expressed at an elevated level in the gut of MED compared to MEAM1, which might be responsible for the MED's higher resistance to insecticides and environmental stresses.Conclusion: The sequencing of MED and MEAM1 gut transcriptomes and extensive comparisons of MEAM1 and MED gut transcripts provide substantial sequence information for revealing the role of gut in whiteflies.
Keyword Gene expression
Genetic divergence
Gut
Transcriptome
Whitefly
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2015 Collection
 
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