Escherichia coli strains expressing H12 antigens demonstrate an increased ability to attach to abiotic surfaces as compared with E. coli strains expressing H7 antigens

Goulter, Rebecca M., Taran, Elena, Gentle, Ian R., Gobius, Kari S. and Dykes, Gary A. (2014) Escherichia coli strains expressing H12 antigens demonstrate an increased ability to attach to abiotic surfaces as compared with E. coli strains expressing H7 antigens. Colloids and Surfaces B: Biointerfaces, 119 90-98. doi:10.1016/j.colsurfb.2014.04.003


Author Goulter, Rebecca M.
Taran, Elena
Gentle, Ian R.
Gobius, Kari S.
Dykes, Gary A.
Title Escherichia coli strains expressing H12 antigens demonstrate an increased ability to attach to abiotic surfaces as compared with E. coli strains expressing H7 antigens
Formatted title
Escherichia coli strains expressing H12 antigens demonstrate an increased ability to attach to abiotic surfaces as compared with E. coli strains expressing H7 antigens
Journal name Colloids and Surfaces B: Biointerfaces   Check publisher's open access policy
ISSN 0927-7765
1873-4367
Publication date 2014-07-01
Sub-type Article (original research)
DOI 10.1016/j.colsurfb.2014.04.003
Open Access Status
Volume 119
Start page 90
End page 98
Total pages 9
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2015
Language eng
Formatted abstract
Highlights
• Loss of FliCH12 in O157:H12 decreased attachment to abiotic surfaces.
• Complementing O157:H12 ΔfliCH12 with fliCH12 restored attachment to wt levels.
ΔfliCH7 strains with complemented fliCH12 attached in higher numbers than wt strains.
FliCH12 and FliCH7 showed different AFM force curve profiles.
• H12 antigens may allow E. coli to attach more readily to abiotic surfaces.

The role of Escherichia coli H antigens in hydrophobicity and attachment to glass, Teflon and stainless steel (SS) surfaces was investigated through construction of fliC knockout mutants in E. coli O157:H7, O1:H7 and O157:H12. Loss of FliCH12 in E. coli O157:H12 decreased attachment to glass, Teflon and stainless steel surfaces (p < 0.05). Complementing E. coli O157:H12 ΔfliCH12 with cloned wildtype (wt) fliCH12 restored attachment to wt levels. The loss of FliCH7 in E. coli O157:H7 and O1:H7 did not always alter attachment (p > 0.05), but complementation with cloned fliCH12, as opposed to cloned fliCH7, significantly increased attachment for both strains compared with wt counterparts (p < 0.05). Hydrophobicity determined using bacterial adherence to hydrocarbons and contact angle measurements differed with fliC expression but was not correlated to the attachment to materials included in this study. Purified FliC was used to functionalise silicone nitride atomic force microscopy probes, which were used to measure adhesion forces between FliC and substrates. Although no significant difference in adhesion force was observed between FliCH12 and FliCH7 probes, differences in force curves suggest different mechanism of attachment for FliCH12 compared with FliCH7. These results indicate that E. coli strains expressing flagellar H12 antigens have an increased ability to attach to certain abiotic surfaces compared with E. coli strains expressing H7 antigens.
Keyword Escherichia coli
Atomic force microscopy
Hydrophobicity
Attachment
H antigens
Flagella
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Fri, 06 Jun 2014, 10:03:15 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences