Light-harvesting complex protein LHCBM9 is critical for photosystem II activity and hydrogen production in Chlamydomonas reinhardtii

Grewe, Sabrina, Ballottari, Matteo, Alcocer, Marcelo, D'Andrea, Cosimo, Blifernez-Klassen, Olga, Hankamer, Ben, Mussgnug, Jan H., Bassi, Roberto and Kruse, Olaf (2014) Light-harvesting complex protein LHCBM9 is critical for photosystem II activity and hydrogen production in Chlamydomonas reinhardtii. Plant Cell, 26 4: 1598-1611. doi:10.1105/tpc.114.124198


Author Grewe, Sabrina
Ballottari, Matteo
Alcocer, Marcelo
D'Andrea, Cosimo
Blifernez-Klassen, Olga
Hankamer, Ben
Mussgnug, Jan H.
Bassi, Roberto
Kruse, Olaf
Title Light-harvesting complex protein LHCBM9 is critical for photosystem II activity and hydrogen production in Chlamydomonas reinhardtii
Formatted title
Light-harvesting complex protein LHCBM9 is critical for photosystem II activity and hydrogen production in Chlamydomonas reinhardtii
Journal name Plant Cell   Check publisher's open access policy
ISSN 1040-4651
1532-298X
Publication date 2014-04-26
Sub-type Article (original research)
DOI 10.1105/tpc.114.124198
Open Access Status
Volume 26
Issue 4
Start page 1598
End page 1611
Total pages 14
Place of publication Rockville, MD, United States
Publisher American Society of Plant Biologists
Collection year 2015
Language eng
Formatted abstract
Photosynthetic organisms developed multiple strategies for balancing light-harvesting versus intracellular energy utilization to survive ever-changing environmental conditions. The light-harvesting complex (LHC) protein family is of paramount importance for this function and can form light-harvesting pigment protein complexes. In this work, we describe detailed analyses of the photosystem II (PSII) LHC protein LHCBM9 of the microalga Chlamydomonas reinhardtii in terms of expression kinetics, localization, and function. In contrast to most LHC members described before, LHCBM9 expression was determined to be very low during standard cell cultivation but strongly increased as a response to specific stress conditions, e.g., when nutrient availability was limited. LHCBM9 was localized as part of PSII supercomplexes but was not found in association with photosystem I complexes. Knockdown cell lines with 50 to 70% reduced amounts of LHCBM9 showed reduced photosynthetic activity upon illumination and severe perturbation of hydrogen production activity. Functional analysis, performed on isolated PSII supercomplexes and recombinant LHCBM9 proteins, demonstrated that presence of LHCBM9 resulted in faster chlorophyll fluorescence decay and reduced production of singlet oxygen, indicating upgraded photoprotection. We conclude that LHCBM9 has a special role within the family of LHCII proteins and serves an important protective function during stress conditions by promoting efficient light energy dissipation and stabilizing PSII supercomplexes.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 04 Jun 2014, 11:18:57 EST by Susan Allen on behalf of Institute for Molecular Bioscience