Tissue expression of the vesicle protein pantophysin

Windoffer, Reinhard, Borchert-Stuhltrager, Monika, Haass, Nikolas K., Thomas, Sabine, Hergt, Michaela, Bulitta, Clemens J. and Leube, R. E. (1999) Tissue expression of the vesicle protein pantophysin. Cell and Tissue Research, 296 3: 499-510. doi:10.1007/s004410051310


Author Windoffer, Reinhard
Borchert-Stuhltrager, Monika
Haass, Nikolas K.
Thomas, Sabine
Hergt, Michaela
Bulitta, Clemens J.
Leube, R. E.
Title Tissue expression of the vesicle protein pantophysin
Journal name Cell and Tissue Research   Check publisher's open access policy
ISSN 0302-766X
1432-0878
Publication date 1999-05
Sub-type Article (original research)
DOI 10.1007/s004410051310
Open Access Status Not Open Access
Volume 296
Issue 3
Start page 499
End page 510
Total pages 12
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Formatted abstract
The cell-type restricted expression of cytoplasmic microvesicle membrane protein isoforms may be a consequence of the functional adaptation of these vesicles to the execution of specialized processes in cells of different specialization. To characterize the expression of the vesicle protein pantophysin, an isoform of the synaptic vesicle proteins synaptophysin and synaptoporin, we have prepared and characterized antibodies useful for the immunological detection of pantophysin in vitro and in situ. Using these reagents, we show by immunoblot analyses that pantophysin expression is not homogeneous but differs significantly between various bovine tissues. Furthermore, these differences are not exactly paralleled by the expression of other vesicle proteins of the SCAMP (secretory carrier-associated membrane protein) and VAMP (vesicle-associated membrane protein) types that have previously been localized to pantophysin vesicles in cultured cells. By immunofluorescence microscopy, pantophysin expression is seen predominantly in non-neuroendocrine cells with pronounced membrane traffic. Pantophysin staining codistributes with SCAMP and VAMP immunoreactivities in most instances but differs in some. Remarkably, pantophysin staining in liver is restricted to cells surrounding sinusoids and is not detectable in hepatocytes, similar to that of the SCAMP and VAMP isoforms as detected by our reagents in tissue sections.
Keyword Vesicle protein
Physin
Secretory carrier-associated membrane protein
Vesicle-associated membrane protein
Synaptobrevin
Expression
Liver
Bovine
Human
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
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Created: Mon, 02 Jun 2014, 00:13:54 EST by Nikolas Haass on behalf of UQ Diamantina Institute