Engineering small MgAl-layered double hydroxide nanoparticles for enhanced gene delivery

Dong, Haiyan, Chen, Min, Rahman, Shafiur, Parekh, Harendra S., Cooper, Helen M. and Xu, Zhi Ping (2014) Engineering small MgAl-layered double hydroxide nanoparticles for enhanced gene delivery. Applied Clay Science, 100 C: 66-75. doi:10.1016/j.clay.2014.04.028

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Author Dong, Haiyan
Chen, Min
Rahman, Shafiur
Parekh, Harendra S.
Cooper, Helen M.
Xu, Zhi Ping
Title Engineering small MgAl-layered double hydroxide nanoparticles for enhanced gene delivery
Journal name Applied Clay Science   Check publisher's open access policy
ISSN 0169-1317
Publication date 2014-10
Sub-type Article (original research)
DOI 10.1016/j.clay.2014.04.028
Open Access Status File (Author Post-print)
Volume 100
Issue C
Start page 66
End page 75
Total pages 10
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2015
Language eng
Abstract In this paper we report an approach for engineering small MgAl-layered double hydroxide (sLDH) nanoparticles with the Z-average diameter of about 40 nm. This method first requires co-precipitation of magnesium and aluminum nitrate solution with sodium hydroxide in methanol, followed by LDH slurry collection and re-suspension in methanol. The methanol suspension is then heated in an autoclave, followed by separation via centrifugation and thorough washing with deionized water. The nanoparticles are finally dispersed in deionized water into homogeneous aqueous suspension after 4–6 day standing at room temperature. In general, sLDH nanoparticles have the Z-average size of 35–50 nm, the number-average size of 14–30 nm and the polydispersity index (PdI) of 0.19–0.25. The prepared sLDH suspension is stable for at least 1 month when stored at fridge (2–8 °C) or ambient (22–25 °C) temperature. Moreover, sLDH nanoparticles are found to carry higher payloads of small double stranded DNA (dsDNA). More excitedly, sLDH nanoparticles transfect dsDNA into HEK 293T cells with a 5 to 6-fold greater efficiency compared to the larger LDH particles (Z-average diameter of 110 nm).
Keyword Small layered double hydroxide nanoparticles
Non-aqueous precipitation and heat-treatment
Ostwald ripening
Gene loading and delivery
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print 14 May 2014

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Created: Thu, 22 May 2014, 10:35:36 EST by Sandrine Ducrot on behalf of Centre for Advanced Imaging