Transcriptional switching in macrophages associated with the peritoneal foreign body response

Mooney, Jane E., Summers, Kim M., Gongora, Milena, Grimmond, Sean M., Campbell, Julie H., Hume, David A. and Rolfe, Barbara E. (2014) Transcriptional switching in macrophages associated with the peritoneal foreign body response. Immunology and Cell Biology, 92 6: 518-526. doi:10.1038/icb.2014.19

Author Mooney, Jane E.
Summers, Kim M.
Gongora, Milena
Grimmond, Sean M.
Campbell, Julie H.
Hume, David A.
Rolfe, Barbara E.
Title Transcriptional switching in macrophages associated with the peritoneal foreign body response
Journal name Immunology and Cell Biology   Check publisher's open access policy
ISSN 0818-9641
Publication date 2014-03-18
Year available 2014
Sub-type Article (original research)
DOI 10.1038/icb.2014.19
Open Access Status
Volume 92
Issue 6
Start page 518
End page 526
Total pages 9
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2015
Language eng
Formatted abstract
We previously demonstrated that myeloid cells are the source of fibrotic tissue induced by foreign material implanted in the peritoneal cavity. This study utilised the MacGreen mouse, in which the Csf1r promoter directs myeloid-specific enhanced green fluorescent protein (EGFP) expression, to determine the temporal gene expression profile of myeloid subpopulations recruited to the peritoneal cavity to encapsulate implanted foreign material (cubes of boiled egg white). Cells with high EGFP expression (EGFPhi) were purified from exudate and encapsulating tissue at different times during the foreign body response, gene expression profiles determined using cDNA microarrays, and data clustered using the network analysis tool, Biolayout Express3D. EGFPhi cells from all time points expressed high levels of Csf1r, Emr1 (encoding F4/80), Cd14 and Itgam (encoding Mac-1) providing internal validation of their myeloid nature. Exudate macrophages (days 4–7) expressed a large cluster of cell cycle genes; these were switched off in capsule cells. Early in capsule formation, Csf1r-EGFPhi cells expressed genes associated with tissue turnover, but later expressed both pro- and anti-inflammatory genes alongside a subset of mesenchyme-associated genes, a pattern of gene expression that adds weight to the concept of a continuum of macrophage phenotypes rather than distinct M1/M2 subsets. Moreover, rather than transdifferentiating to myofibroblasts, macrophages contributing to later stages of the peritoneal foreign body response warrant their own classification as ‘fibroblastoid’ macrophages. 
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print 18 March 2014.

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Created: Wed, 21 May 2014, 12:58:50 EST by Susan Allen on behalf of Institute for Molecular Bioscience