The murine gammaherpesvirus 68 ORF27 gene product contributes to intercellular viral spread

May, Janet S., Walker, Jennifer, Colaco, Susanna and Stevenson, Philip G. (2005) The murine gammaherpesvirus 68 ORF27 gene product contributes to intercellular viral spread. Journal of Virology, 79 8: 5059-5068. doi:10.1128/JVI.79.8.5059-5068.2005


Author May, Janet S.
Walker, Jennifer
Colaco, Susanna
Stevenson, Philip G.
Title The murine gammaherpesvirus 68 ORF27 gene product contributes to intercellular viral spread
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
1098-5514
Publication date 2005-04-01
Year available 2005
Sub-type Article (original research)
DOI 10.1128/JVI.79.8.5059-5068.2005
Open Access Status DOI
Volume 79
Issue 8
Start page 5059
End page 5068
Total pages 10
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Subject 2403 Immunology
Abstract Herpesviruses remain predominantly cell associated within their hosts, implying that they spread between cells by a mechanism distinct from free virion release. We previously identified the efficient release of murine gammaherpesvirus 68 (MHV-68) virions as a function of the viral gp150 protein. Here we show that the MHV-68 ORF27 gene product, gp48, contributes to the direct spread of viruses from lytically infected to uninfected cells. Monoclonal antibodies to gp48 identified it on infected cell surfaces and in virions. gp48-deficient viruses showed no obvious deficit in virion cell binding, single-cycle replication, or virion release but had reduced lytic propagation between cells. After intranasal infection of mice, ORF27-deficient viruses were impaired predominantly in lytic replication in the lungs. There was a small deficit in latency establishment, but long-term latency appeared normal. Since ORF27 has homologs in both Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus, it is likely part of a conserved mechanism employed by gammaherpesviruses to disseminate lytically in their hosts. Copyright
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: ResearcherID Downloads
Scopus Import
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 31 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 31 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 21 May 2014, 22:45:26 EST by System User on behalf of Scholarly Communication and Digitisation Service