A mechanistic basis for potent, glycoprotein B-directed gammaherpesvirus neutralization

Glauser, Daniel L., Kratz, Anne-Sophie, Gillet, Laurent and Stevenson, Philip G. (2011) A mechanistic basis for potent, glycoprotein B-directed gammaherpesvirus neutralization. Journal of General Virology, 92 9: 2020-2033. doi:10.1099/vir.0.032177-0


Author Glauser, Daniel L.
Kratz, Anne-Sophie
Gillet, Laurent
Stevenson, Philip G.
Title A mechanistic basis for potent, glycoprotein B-directed gammaherpesvirus neutralization
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 0022-1317
1465-2099
Publication date 2011
Sub-type Article (original research)
DOI 10.1099/vir.0.032177-0
Open Access Status
Volume 92
Issue 9
Start page 2020
End page 2033
Total pages 14
Place of publication Reading, Berks, United Kingdom
Publisher Society for General Microbiology
Language eng
Subject 2406 Virology
Abstract Glycoprotein B (gB) is a conserved, essential component of gammaherpes virions and so potentially vulnerable to neutralization. However, few good gB-specific neutralizing antibodies have been identified. Here, we show that murid herpesvirus 4 is strongly neutralized by mAbs that recognize an epitope close to one of the gB fusion loops. Antibody binding did not stop gB interacting with its cellular ligands or initiating its fusion-associated conformation change, but did stop gB resolving stably to its post-fusion form, and so blocked membrane fusion to leave virions stranded in late endosomes. The conservation of gB makes this mechanism a possible general route to gammaherpesvirus neutralization.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Clinical Medical Virology Centre Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 10 times in Scopus Article | Citations
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