MHC class I ubiquitination by a viral PHD/LAP finger protein

Boname, Jessica M. and Stevenson, Philip G. (2001) MHC class I ubiquitination by a viral PHD/LAP finger protein. Immunity, 15 4: 627-636. doi:10.1016/S1074-7613(01)00213-8

Author Boname, Jessica M.
Stevenson, Philip G.
Title MHC class I ubiquitination by a viral PHD/LAP finger protein
Journal name Immunity   Check publisher's open access policy
ISSN 1074-7613
Publication date 2001-10
Sub-type Article (original research)
DOI 10.1016/S1074-7613(01)00213-8
Open Access Status Not yet assessed
Volume 15
Issue 4
Start page 627
End page 636
Total pages 10
Place of publication Cambridge, MA, United States
Publisher Cell Press
Language eng
Formatted abstract
The murine γ-herpesvirus-68 K3 (MK3) is a PHD/LAP finger protein that downregulates major histocompatibility complex (MHC) class I expression. In transfected cell lines, MK3 was expressed in the endoplasmic reticulum (ER) membrane, where it bound the cytoplasmic tail of newly synthesized H-2Db glycoproteins and targeted them for degradation. Proteasome inhibitors blocked the degradation and led to an accumulation of ubiquitinated H-2Db. Because this retained its native conformation, ubiquitination preceded any denaturation or dislocation to the cytosol. The PHD/LAP finger of MK3 was not required for H-2Db binding but was essential for its ubiquitination and degradation. Thus, γ-herpesviruses have adapted the cellular PHD/LAP motif to immune evasion, apparently for the catalysis of MHC class I ubiquitination.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 153 times in Thomson Reuters Web of Science Article | Citations
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