An essential role for the proximal but not the distal cytoplasmic tail of glycoprotein M in murid herpesvirus 4 infection

May, Janet S., Smith, Christopher M., Gill, Michael B. and Stevenson, Philip G. (2008) An essential role for the proximal but not the distal cytoplasmic tail of glycoprotein M in murid herpesvirus 4 infection. PLoS ONE, 3 5: e2131.1-e2131.11. doi:10.1371/journal.pone.0002131


Author May, Janet S.
Smith, Christopher M.
Gill, Michael B.
Stevenson, Philip G.
Title An essential role for the proximal but not the distal cytoplasmic tail of glycoprotein M in murid herpesvirus 4 infection
Journal name PLoS ONE   Check publisher's open access policy
ISSN 1932-6203
Publication date 2008-05-07
Year available 2008
Sub-type Article (original research)
DOI 10.1371/journal.pone.0002131
Open Access Status DOI
Volume 3
Issue 5
Start page e2131.1
End page e2131.11
Total pages 11
Place of publication San Francisco United States
Publisher Public Library of Science (PLoS)
Language eng
Abstract Murid herpesvirus-4 (MuHV-4) provides a tractable model with which to define common, conserved features of gamma-herpesvirus biology. The multi-membrane spanning glycoprotein M (gM) is one of only 4 glycoproteins that are essential for MuHV-4 lytic replication. gM binds to gN and is thought to function mainly secondary envelopment and virion egress, for which several predicted trafficking motifs in its C-terminal cytoplasmic tail could be important. We tested the contribution of the gM cytoplasmic tail to MuHV-4 lytic replication by making recombinant viruses with varying C-terminal deletions. Removing an acidic cluster and a distal YXXΦ motif altered the capsid distribution somewhat in infected cells but had little effect on virus replication, either in vitro or in vivo. In contrast, removing a proximal YXXΦ motif as well completely prevented productive replication. gM was still expressed, but unlike its longer forms showed only limited colocalization with co-transfected gN, and in the context of whole virus appeared to support gN expression less well. We conclude that some elements of the gM cytoplasmic tail are dispensible for MuHV-4 replication, but the tail as a whole is not.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Clinical Medical Virology Centre Publications
 
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