Comparison of the innate immune responses of porcine monocyte-derived dendritic cells and splenic dendritic cells stimulated with LPS

Qu, Xueqi, Cinar, Mehmet U., Fan, Huitao, Pröll, Maren, Tesfaye, Dawit, Tholen, Ernst, Looft, Christian, Hölker, Michael, Schellander, Karl and Uddin, Muhammad J. (2014) Comparison of the innate immune responses of porcine monocyte-derived dendritic cells and splenic dendritic cells stimulated with LPS. Innate Immunity, OnlineFirst 1-14. doi:10.1177/1753425914526266


Author Qu, Xueqi
Cinar, Mehmet U.
Fan, Huitao
Pröll, Maren
Tesfaye, Dawit
Tholen, Ernst
Looft, Christian
Hölker, Michael
Schellander, Karl
Uddin, Muhammad J.
Title Comparison of the innate immune responses of porcine monocyte-derived dendritic cells and splenic dendritic cells stimulated with LPS
Journal name Innate Immunity   Check publisher's open access policy
ISSN 1753-4259
1753-4267
Publication date 2014-03-19
Sub-type Article (original research)
DOI 10.1177/1753425914526266
Open Access Status
Volume OnlineFirst
Start page 1
End page 14
Total pages 14
Place of publication London, United Kingdom
Publisher Sage Publications
Collection year 2015
Language eng
Formatted abstract
Dendritic cell (DC) subsets form a remarkable cellular network that regulate innate and adaptive immune responses. Although pigs are the most approximate model to humans, little is known about the regulation of monocyte-derived DCs (moDCs) and splenic DCs (SDCs) in the initiation of immune responses under inflammatory conditions. We investigated the activation and maturation of porcine moDC and SDC subpopulations following LPS stimulation. Porcine monocytes that would differentiate into moDCs were isolated. SDCs were isolated directly from the porcine spleen. Following LPS stimulation, phagocytosis activity, TLR4/MyD88-dependent gene expression, co-stimulatory molecule, and pro-inflammatory cytokine (TNF-α, IL-1β) and chemokine (IL-8) expressions were increased in both cell subsets. Furthermore, moDCs showed higher levels of gene and protein expression compared with SDCs. Interestingly, moDCs were found to be more responsive via the TLR4/TRAF-dependent signalling pathway of activation. Only SDCs expressed higher level of IL-12p40 gene and protein, whereas, IFN-γ gene and protein expression were likely to be unchanged after LPS stimulation in both cell subtypes. These data demonstrate that porcine moDCs display a greater ability to initiate innate immune responses, and could be used as a model to investigate immune responses against Ags.
Keyword APCs
Pigs
LPS
TLRs
Cytokines
Pattern recognition receptors
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Published online before print March 19, 2014

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Veterinary Science Publications
 
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Created: Tue, 20 May 2014, 11:13:40 EST by Jasim Uddin on behalf of School of Veterinary Science