Characterization of trifluralin binding with recombinant tubulin from Trypanosoma brucei

Giles, Natalie L., Armson, Anthony and Reid, Simon A. (2009) Characterization of trifluralin binding with recombinant tubulin from Trypanosoma brucei. Parasitology Research, 104 4: 893-903. doi:10.1007/s00436-008-1271-2


Author Giles, Natalie L.
Armson, Anthony
Reid, Simon A.
Title Characterization of trifluralin binding with recombinant tubulin from Trypanosoma brucei
Formatted title
Characterization of trifluralin binding with recombinant tubulin from Trypanosoma brucei 
Journal name Parasitology Research   Check publisher's open access policy
ISSN 0932-0113
1432-1955
Publication date 2009
Sub-type Article (original research)
DOI 10.1007/s00436-008-1271-2
Open Access Status
Volume 104
Issue 4
Start page 893
End page 903
Total pages 11
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Abstract The binding kinetics of five novel trifluralin analogs with recombinant α- and β-tubulin proteins from Trypanosoma brucei rhodesiense was determined. Native tubulin from rats was used to determine the extent of binding of each analog to mammalian tubulin. The results of this study clearly demonstrate two important characteristics of the binding of these trifluralins to tubulin. Firstly, they have specific affinity for trypanosomal tubulin compared with mammalian tubulin irrespective of the chemical composition of the trifluralin analog tested. Secondly, they have a stronger affinity for trypanosomal α-tubulin compared with trypanosomal β-tubulin. In addition, compounds 1007, 1008, 1016, and 1017 have strong binding affinities for α-tubulin, with limited binding affinity for mammalian tubulin, which indicates that these compounds selectively bind to trypanosomal tubulin.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Public Health Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 10 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 20 May 2014, 11:07:47 EST by System User on behalf of School of Public Health