Immunization with recombinant beta-tubulin from Trypanosoma evansi induced protection against T. evansi, T. equiperdum and T. b. brucei infection in mice

Li S.-Q., Fung M.-C., Reid S.A., Inoue N. and Lun Z.-R. (2007) Immunization with recombinant beta-tubulin from Trypanosoma evansi induced protection against T. evansi, T. equiperdum and T. b. brucei infection in mice. Parasite Immunology, 29 4: 191-199. doi:10.1111/j.1365-3024.2006.00933.x


Author Li S.-Q.
Fung M.-C.
Reid S.A.
Inoue N.
Lun Z.-R.
Title Immunization with recombinant beta-tubulin from Trypanosoma evansi induced protection against T. evansi, T. equiperdum and T. b. brucei infection in mice
Journal name Parasite Immunology   Check publisher's open access policy
ISSN 0141-9838
Publication date 2007-01-01
Sub-type Article (original research)
DOI 10.1111/j.1365-3024.2006.00933.x
Volume 29
Issue 4
Start page 191
End page 199
Total pages 9
Language eng
Subject 2403 Immunology
2405 Parasitology
Abstract The beta-tubulin gene of Trypanosoma evansi (STIB 806) was cloned and expressed in Escherichia coli. The predicted amino acid sequence of T. evansi beta-tubulin shows 100%, 99·8%, 99·1%, and 98·6% homology with T. equiperdum, T. b. brucei, T. cruzi and T. danilewskyi, respectively, but is diverse from that of T. cyclops, showing only 51·6% of homology. Recombinant beta-tubulin was expressed as inclusion bodies in E. coli. It was purified and renatured for immunological studies. Mice immunized with the renatured recombinant beta-tubulin were protected from lethal challenge with T. evansi STIB 806, T. equiperdum STIB 818 and T. b. brucei STIB 940, showing 83·3%, 70% and 76·7% protection, respectively. Serum collected from the rabbit immunized with recombinant beta-tubulin inhibited the growth of T. evansi, T. equiperdum and T. b. brucei in vitro. Serum from mice and rabbits immunized with recombinant beta-tubulin recognized only T. evansi beta-tubulin and not mouse beta-tubulin. The results of this study demonstrated that the recombinant T. evansi beta-tubulin is a potential candidate for the development of a vaccine to prevent animal trypanosomiasis caused by these three trypanosome species.
Keyword Beta-tubulin
Recombinant
T. b. brucei
T. equiperdum
Trypanosoma evansi
Vaccine
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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