Differential expression of functional nucleoside transporters in non-differentiated and differentiated human endothelial progenitor cells

Guzman-Gutierrez, E., Sandoval, C., Nova, E., Castillo, J. L., Vera, J. C., Lamperti, L., Krause, B., Salomon, C., Sepulveda, C., Aguayo, C. and Sobrevia, L. (2010) Differential expression of functional nucleoside transporters in non-differentiated and differentiated human endothelial progenitor cells. Placenta, 31 10: 928-936. doi:10.1016/j.placenta.2010.07.016


Author Guzman-Gutierrez, E.
Sandoval, C.
Nova, E.
Castillo, J. L.
Vera, J. C.
Lamperti, L.
Krause, B.
Salomon, C.
Sepulveda, C.
Aguayo, C.
Sobrevia, L.
Title Differential expression of functional nucleoside transporters in non-differentiated and differentiated human endothelial progenitor cells
Journal name Placenta   Check publisher's open access policy
ISSN 0143-4004
1532-3102
Publication date 2010
Sub-type Article (original research)
DOI 10.1016/j.placenta.2010.07.016
Open Access Status
Volume 31
Issue 10
Start page 928
End page 936
Total pages 9
Place of publication London, United Kingdom
Publisher Elsevier
Language eng
Abstract Extracellular adenosine removal is via human equilibrative nucleoside transporters 1 (hENT1) and 2 (hENT2) in the endothelium, thus regulating adenosine-induced revascularization and angiogenesis. Since human endothelial progenitor cells (hEPCs) promote revascularization, we hypothesize differential expression of nucleoside transporters in hEPCs. hEPCs were cultured 3 (hEPC-3d) or 14 (hEPC-14d) days. RT-PCR for prominin 1, CD34, octamer-4, kinase insert domain receptor, oxidized low-density lipoprotein (lectin-like) receptor 1 and tyrosine endothelial kinase was used to evaluate phenotypic differentiation. Flow cytometry was used to estimate CD34+/KDR- (non-differentiated), CD34-/KDR+ (differentiated) or CD34+/KDR+ (mixed) cell populations. Adenosine transport was measured in absence or presence of sodium, S-(4-nitrobenzyl)-6-thio-inosine (NBTI, 1-10 μM), inosine, hypoxanthine or guanine (0.1-5 mM), hENTs protein abundance by western blot, and hENTs, hCNT1, hCNT2 and hCNT3 mRNA expression by real time RT-PCR. hEPC-3d cells were CD34+/KDR- compared with hEPC-14d cells that were CD34-/KDR+. hEPC-3d cells exhibit hENT1-like adenosine transport (NBTI-sensitive, Na+- independent), which is absent in hEPC-14d cells. hEPC-14d cells exhibit two transport components: component 1 (NBTI insensitive, Na+-independent) and component 2 (NBTI insensitive, Na+-dependent, Hill coefficient ∼1.8), the latter resembling CNT3-like transport. hEPC-3d cells express hENT1 protein and mRNA, which is reduced (∼90%) in hEPC-14d cells, but instead only hCNT3 mRNA is expressed in this cell type. hENT2, hCNT1 and hCNT2 were undetectable in hEPCs. Thus, hEPCs exhibit a differential expression of hENT1 and hCNT3 functional nucleoside transporters, which could be related with its differentiation stage.
Keyword Adenosine
Differentiation
Endothelial progenitor cells
Transport
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 8 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 20 May 2014, 11:02:42 EST by System User on behalf of UQ Centre for Clinical Research