PTEN hypermethylation profiles of Chinese Kazakh patients with esophageal squamous cell carcinoma

Pan, Q. F., Li, W. T., Dong, H. C., Chen, Y. Z., Yin, L., Liu, W., Wang, W. W., Liu, D., Li, S. G., Gu, W. Y., Chen, J. Z., Yang, L., Zhang, W. J. and Li, F. (2014) PTEN hypermethylation profiles of Chinese Kazakh patients with esophageal squamous cell carcinoma. Diseases of the Esophagus, 27 4: 396-402. doi:10.1111/dote.12106


Author Pan, Q. F.
Li, W. T.
Dong, H. C.
Chen, Y. Z.
Yin, L.
Liu, W.
Wang, W. W.
Liu, D.
Li, S. G.
Gu, W. Y.
Chen, J. Z.
Yang, L.
Zhang, W. J.
Li, F.
Title PTEN hypermethylation profiles of Chinese Kazakh patients with esophageal squamous cell carcinoma
Formatted title
PTEN hypermethylation profiles of Chinese Kazakh patients with esophageal squamous cell carcinoma
Journal name Diseases of the Esophagus   Check publisher's open access policy
ISSN 1442-2050
1120-8694
Publication date 2014-05
Year available 2014
Sub-type Article (original research)
DOI 10.1111/dote.12106
Open Access Status
Volume 27
Issue 4
Start page 396
End page 402
Total pages 7
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing Asia
Collection year 2015
Language eng
Formatted abstract
Aberrant DNA methylation of promoter region CpG islands may serve as an alternative mechanism to genetic defects in the inactivation of tumor suppressor genes (TSGs) in human malignancies. The aim of this study was to examine the promoter methylation status of the PTENTSG and its association with esophageal squamous cell carcinoma (ESCC) carcinogenesis in a Chinese Kazakh population, which is known to have a relatively high ESCC incidence and mortality. The methylation status of the PTEN promoter region was determined in patients with ESCC (n = 95) and healthy individuals (n = 65) using highly sensitive Sequenom Epityper assays. The methylation level of the PTEN gene was significantly higher in patients with ESCC than in healthy controls. The median methylation level was 10.0% (interquartile range [IQR]: 7.0-11.0%) in patients with ESCC and 6.0% in controls (IQR: 4.0-9.0%; P = 0.001). PTEN methylation levels were higher in male patients with ESCC than in male controls, whereas a trend toward significance was observed between female patients with ESCC and female controls (P = 0.005 and P = 0.086, respectively). The PTEN methylation level was associated with histopathological grade and lymph node metastasis in patients with ESCC (P = 0.002 and P = 0.009, respectively). To our knowledge, this is the first report to show the presence of PTEN promoter CpG hypermethylation in ESCC and its association with tumor metastasis.
Keyword Esophageal carcinoma
MALDI-TOF
Promoter methylation
PTEN gene
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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