Cryptic epitopes of albumin determine mononuclear phagocyte system clearance of nanomaterials

Mortimer, Gysell M., Butcher, Neville J., Musumeci, Anthony W., Deng, Zhou J., Martin, Darren J. and Minchin, Rodney F. (2014) Cryptic epitopes of albumin determine mononuclear phagocyte system clearance of nanomaterials. ACS Nano, 8 4: 3357-3366. doi:10.1021/nn405830g


Author Mortimer, Gysell M.
Butcher, Neville J.
Musumeci, Anthony W.
Deng, Zhou J.
Martin, Darren J.
Minchin, Rodney F.
Title Cryptic epitopes of albumin determine mononuclear phagocyte system clearance of nanomaterials
Journal name ACS Nano   Check publisher's open access policy
ISSN 1936-0851
1936-086X
Publication date 2014-04-22
Year available 2014
Sub-type Article (original research)
DOI 10.1021/nn405830g
Open Access Status DOI
Volume 8
Issue 4
Start page 3357
End page 3366
Total pages 10
Place of publication Washington, DC United States
Publisher American Chemical Society
Collection year 2015
Language eng
Subject 2200 Engineering
2500 Materials Science
3100 Physics and Astronomy
Abstract While plasma proteins can influence the physicochemical properties of nanoparticles, the adsorption of protein to the surface of nanomaterials can also alter the structure and function of the protein. Here, we show that plasma proteins form a hard corona around synthetic layered silicate nanoparticles (LSN) and that one of the principle proteins is serum albumin. The protein corona was required for recognition of the nanoparticles by scavenger receptors, a major receptor family associated with the mononuclear phagocyte system (MPS). Albumin alone could direct nanoparticle uptake by human macrophages, which involved class A but not class B scavenger receptors. Upon binding to LSN, albumin unfolded to reveal a cryptic epitope that could also be exposed by heat denaturation. This work provides an understanding of how albumin, and possibly other proteins, can promote nanomaterial recognition by the MPS without albumin requiring chemical modification for scavenger receptor recognition. These findings also demonstrate an additional function for albumin in vivo.
Keyword Albumin
Layered silicate nanoparticles
Mononuclear Phagocyte System
Protein corona
Scavenger Receptor
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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