Global quantification of tissue dynamics in the developing mouse kidney

Short, Kieran M., Combes, Alexander N., Lefevre, James, Ju, Adler L., Georgas, Kylie M., Lamberton, Timothy, Cairncross, Oliver, Rumballe, Bree A., McMahon, Andrew P., Hamilton, Nicholas A., Smyth, Ian M. and Little, Melissa H. (2014) Global quantification of tissue dynamics in the developing mouse kidney. Developmental Cell, 29 2: 188-202. doi:10.1016/j.devcel.2014.02.017


Author Short, Kieran M.
Combes, Alexander N.
Lefevre, James
Ju, Adler L.
Georgas, Kylie M.
Lamberton, Timothy
Cairncross, Oliver
Rumballe, Bree A.
McMahon, Andrew P.
Hamilton, Nicholas A.
Smyth, Ian M.
Little, Melissa H.
Title Global quantification of tissue dynamics in the developing mouse kidney
Journal name Developmental Cell   Check publisher's open access policy
ISSN 1878-1551
1534-5807
Publication date 2014-04-28
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.devcel.2014.02.017
Open Access Status DOI
Volume 29
Issue 2
Start page 188
End page 202
Total pages 15
Place of publication Cambridge, MA, United States
Publisher Cell Press
Collection year 2015
Language eng
Abstract Although kidneys of equal size can vary 10-fold in nephron number at birth, discovering what regulates such variation has been hampered by a lack of quantitative parameters defining kidney development. Here we report a comprehensive, quantitative, multiscale analysis of mammalian kidney development in which we measure changes in cell number, compartment volumes, and cellular dynamics across the entirety of organogenesis, focusing on two key nephrogenic progenitor populations: the ureteric epithelium and the cap mesenchyme. In doing so, we describe a discontinuous developmental program governed by dynamic changes in interactions between these key cellular populations occurring within a previously unappreciated structurally stereotypic organ architecture. We also illustrate the application of this approach to the detection of a subtle mutant phenotype. This baseline program of kidney morphogenesis provides a framework for assessing genetic and environmental developmental perturbation and will serve as a gold standard for the analysis of other organs.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Institute for Molecular Bioscience - Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 34 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 39 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 20 May 2014, 00:27:37 EST by System User on behalf of Institute for Molecular Bioscience