Curcumin-cyclodextrin encapsulated chitosan nanoconjugates with enhanced solubility and cell cytotoxicity

Popat, Amirali, Karmakar, Surajit, Jambhrunkar, Siddharth, Xu, Chun and Yu, Chengzhong (2014) Curcumin-cyclodextrin encapsulated chitosan nanoconjugates with enhanced solubility and cell cytotoxicity. Colloids and Surfaces B: Biointerfaces, 117 520-527. doi:10.1016/j.colsurfb.2014.03.005

Author Popat, Amirali
Karmakar, Surajit
Jambhrunkar, Siddharth
Xu, Chun
Yu, Chengzhong
Title Curcumin-cyclodextrin encapsulated chitosan nanoconjugates with enhanced solubility and cell cytotoxicity
Journal name Colloids and Surfaces B: Biointerfaces   Check publisher's open access policy
ISSN 0927-7765
Publication date 2014-05-01
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.colsurfb.2014.03.005
Open Access Status
Volume 117
Start page 520
End page 527
Total pages 8
Place of publication Amsterdam, The Netherlands
Publisher Elsevier
Collection year 2015
Language eng
Abstract Curcumin (CUR), a naturally derived anti-cancer cocktail is arguably the most widely studied neutraceutical. Despite a lot of promises, it is yet to reach the market as an active anti-cancer formulation. In the present study, we have prepared highly soluble (3. mg/ml) CUR-γ-hydroxypropyl cyclodextrin (CUR-CD) hollow spheres. CUR-CD hollow spheres were prepared by a novel and scalable spray drying method. CUR-CD was then encapsulated into positively charged biodegradable chitosan (CUR-CD-CS) nanoparticles. The CUR-CD-CS nanoparticles were characterised by TEM, SEM, DLS, drug loading and in vitro release. We tested the efficacy of these CUR-CD-CS nanoparticles in SCC25 cell lines using MTT assay and investigated its cellular uptake mechanism. We also studied Oligo DNA loading in CUR-CD-CS nanoparticles and its delivery via confocal imaging and FACS analysis. Our results demonstrated that CUR-CD-CS nanoparticles showed superior in vitro release performance and higher cytotoxicity in SCC25 cell line amongst all tested formulations. The cytotoxicity results were corroborated by cell cycle analysis and apoptosis test, showing nearly 100% apoptotic cell death in the case of CUR-CD-CS nanoparticles. Compared to CS nanoparticles, CS-CD nanoformulation showed higher cellular delivery of Cy3-Oligo DNA which was tested quantitatively using flowcytometry analysis, indicating that CD not only enhanced CUR solubility but also boosted the cellular uptake. Our study shows that rationally designed bio-degradable natural biomaterials have great potential as next generation nano-carriers for hydrophobic drug delivery such as CUR with potential of dual drug-gene delivery.
Keyword Cellular delivery
Spray drying
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

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