Analysis of bulge stem cells from the epidermis using flow cytometry

Villani, Rehan M., Akyuz, Mehmet Deniz, Niessen, Michaela T. and Niessen, Carien M. (2013). Analysis of bulge stem cells from the epidermis using flow cytometry. In Kursad Turksen (Ed.), Skin stem cells (pp. 33-43) New York, United States: Humana Press. doi:10.1007/978-1-62703-330-5_4

Author Villani, Rehan M.
Akyuz, Mehmet Deniz
Niessen, Michaela T.
Niessen, Carien M.
Title of chapter Analysis of bulge stem cells from the epidermis using flow cytometry
Title of book Skin stem cells
Place of Publication New York, United States
Publisher Humana Press
Publication Year 2013
Sub-type Research book chapter (original research)
DOI 10.1007/978-1-62703-330-5_4
Open Access Status
Series Methods in Molecular Biology
ISBN 9781627033299
ISSN 1064-3745
Editor Kursad Turksen
Volume number 989
Chapter number 4
Start page 33
End page 43
Total pages 11
Total chapters 24
Collection year 2014
Language eng
Abstract/Summary The epidermis is a multilayered epithelium consisting of multiple different progenitor cell populations, all of which are important to epidermal function. In order to study these populations, several techniques have been developed that enable specific purification of the different progenitor cell populations. The best characterized stem cell population in the epidermis, and likely the most pluripotent, are the quiescent stem cells in the hair follicle bulge. In this chapter, we provide a method for isolating bulge stem cells from skin of adult mice using fluorescence-activated cell sorting of immunofluorescently labeled keratinocytes. We use the cell surface markers CD34 and α6-integrin for the enrichment of bulge stem cells. This method also contains notes on how to adjust the cytometer settings for a reproducible analysis.
Keyword α6-Integrin
Bulge stem cells
Epithelial stem cells
Fluorescence-activated cell sorting
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Book Chapter
Collections: Non HERDC
UQ Diamantina Institute Publications
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Created: Mon, 19 May 2014, 12:09:58 EST by Rehan Villani on behalf of UQ Diamantina Institute