SNP markers-based map construction and genome-wide linkage analysis in Brassica napus

Raman, Harsh, Dalton-Morgan, Jessica, Diffey, Simon, Raman, Rosy, Alamery, Salman, Edwards, David and Batley, Jacqueline (2014) SNP markers-based map construction and genome-wide linkage analysis in Brassica napus. Plant Biotechnology Journal, 12 7: 851-860. doi:10.1111/pbi.12186


Author Raman, Harsh
Dalton-Morgan, Jessica
Diffey, Simon
Raman, Rosy
Alamery, Salman
Edwards, David
Batley, Jacqueline
Title SNP markers-based map construction and genome-wide linkage analysis in Brassica napus
Formatted title
SNP markers-based map construction and genome-wide linkage analysis in Brassica napus
Journal name Plant Biotechnology Journal   Check publisher's open access policy
ISSN 1467-7644
1467-7652
Publication date 2014-04-03
Sub-type Article (original research)
DOI 10.1111/pbi.12186
Open Access Status DOI
Volume 12
Issue 7
Start page 851
End page 860
Total pages 10
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell
Collection year 2015
Language eng
Formatted abstract
An Illumina Infinium array comprising 5306 single nucleotide polymorphism (SNP) markers was used to genotype 175 individuals of a doubled haploid population derived from a cross between Skipton and Ag-Spectrum, two Australian cultivars of rapeseed (Brassica napus L.). A genetic linkage map based on 613 SNP and 228 non-SNP (DArT, SSR, SRAP and candidate gene markers) covering 2514.8 cM was constructed and further utilized to identify loci associated with flowering time and resistance to blackleg, a disease caused by the fungus Leptosphaeria maculans. Comparison between genetic map positions of SNP markers and the sequenced Brassica rapa (A) and Brassica oleracea (C) genome scaffolds showed several genomic rearrangements in the B. napus genome. A major locus controlling resistance to L. maculans was identified at both seedling and adult plant stages on chromosome A07. QTL analyses revealed that up to 40.2% of genetic variation for flowering time was accounted for by loci having quantitative effects. Comparative mapping showed Arabidopsis and Brassica flowering genes such as Phytochrome A/D, Flowering Locus C and agamous-Like MADS box gene AGL1 map within marker intervals associated with flowering time in a DH population from Skipton/Ag-Spectrum. Genomic regions associated with flowering time and resistance to L. maculans had several SNP markers mapped within 10 cM. Our results suggest that SNP markers will be suitable for various applications such as trait introgression, comparative mapping and high-resolution mapping of loci in B. napus
Keyword Genetic Linkage Mapping
Single nucleotide polymorphism (SNP)
Quantitative trait loci (QTLs)
Blackleg resistance
Flowering time
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2015 Collection
 
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Created: Thu, 15 May 2014, 22:29:44 EST by Jessica Dalton-Morgan on behalf of School of Agriculture and Food Sciences