New technologies for ultrahigh-throughput genotyping in plant taxonomy

Edwards, David, Zander, Manuel, Dalton-Morgan, Jessica and Batley, Jacqueline (2014). New technologies for ultrahigh-throughput genotyping in plant taxonomy. In Pascale Besse (Ed.), Molecular Plant Taxonomy: Methods and Protocols (pp. 151-175) New York, NY, United States: Humana Press. doi:10.1007/978-1-62703-767-9_8

Author Edwards, David
Zander, Manuel
Dalton-Morgan, Jessica
Batley, Jacqueline
Title of chapter New technologies for ultrahigh-throughput genotyping in plant taxonomy
Title of book Molecular Plant Taxonomy: Methods and Protocols
Place of Publication New York, NY, United States
Publisher Humana Press
Publication Year 2014
Sub-type Research book chapter (original research)
DOI 10.1007/978-1-62703-767-9_8
Open Access Status
Series Methods in Molecular Biology
ISBN 9781627037662
ISSN 1064-3745
Editor Pascale Besse
Volume number 1115
Chapter number 8
Start page 151
End page 175
Total pages 25
Total chapters 18
Collection year 2015
Language eng
Formatted Abstract/Summary
Molecular genetic markers represent one of the most powerful tools for the analysis of variation between plant genomes. Molecular marker technology has developed rapidly over the last decade, with the introduction of new DNA sequencing methods and the development of high-throughput genotyping methods. Single nucleotide polymorphisms (SNPs) now dominate applications in modern plant genetic analysis. The reducing cost of DNA sequencing and increasing availability of large sequence data sets permit the mining of this data for large numbers of SNPs. These may then be used in applications such as genetic linkage analysis and trait mapping, diversity analysis, association studies, and marker-assisted selection. Here we describe automated methods for the discovery of SNP molecular markers and new technologies for high-throughput, low-cost molecular marker genotyping. Examples include SNP discovery using autoSNPdb and as well as SNP genotyping using Illumina’s GoldenGate™ and Infinium™ methods.
Keyword AutoSNP
Infinium (TM)
Single nucleotide polymorphism
SNPlex (TM)
Q-Index Code BX
Q-Index Status Confirmed Code
Institutional Status UQ

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Created: Thu, 15 May 2014, 09:57:10 EST by Jessica Dalton-Morgan on behalf of School of Agriculture and Food Sciences