Flow cytometric-membrane potential detection of sodium channel active marine toxins: application to ciguatoxins in fish muscle and feasibility of automating saxitoxin detection

Manger, Ronald, Woodle, Doug, Berger, Andrew, Dickey, Robert W., Jester, Edward, Yasumoto, Takesh, Lewis, Richard, Hawryluk, Timothy and Hungerford, James (2014) Flow cytometric-membrane potential detection of sodium channel active marine toxins: application to ciguatoxins in fish muscle and feasibility of automating saxitoxin detection. Journal of AOAC International, 97 2: 299-306. doi:10.5740/jaoacint.SGEManger

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Author Manger, Ronald
Woodle, Doug
Berger, Andrew
Dickey, Robert W.
Jester, Edward
Yasumoto, Takesh
Lewis, Richard
Hawryluk, Timothy
Hungerford, James
Title Flow cytometric-membrane potential detection of sodium channel active marine toxins: application to ciguatoxins in fish muscle and feasibility of automating saxitoxin detection
Journal name Journal of AOAC International   Check publisher's open access policy
ISSN 1060-3271
1944-7922
Publication date 2014
Year available 2014
Sub-type Article (original research)
DOI 10.5740/jaoacint.SGEManger
Open Access Status
Volume 97
Issue 2
Start page 299
End page 306
Total pages 8
Place of publication Rockville, MD, United States
Publisher AOAC International
Collection year 2015
Language eng
Abstract Ciguatoxins are potent neurotoxins with a significant public health impact. Cytotoxicity assays have allowed the most sensitive means of detection of ciguatoxin-like activity without reliance on mouse bioassays and have been invaluable in studying outbreaks. An improvement of these cell-based assays is presented here in which rapid flow cytometric detection of ciguatoxins and saxitoxins is demonstrated using fluorescent voltage sensitive dyes. A depolarization response can be detected directly due to ciguatoxin alone; however, an approximate 1000-fold increase in sensitivity is observed in the presence of veratridine. These results demonstrate that flow cytometric assessment of ciguatoxins is possible at levels approaching the trace detection limits of our earlier cytotoxicity assays, however, with a significant reduction in analysis time. Preliminary results are also presented for detection of brevetoxins and for automation and throughput improvements to a previously described method for detecting saxitoxins in shellfish extracts.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Institute for Molecular Bioscience - Publications
 
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