In search of a novel target - Phosphatidylserine exposed by non-apoptotic tumor cells and metastases of malignancies with poor treatment efficacy

Riedl, Sabrina, Rinner, Beate, Asslaber, Martin, Schaider, Helmut, Walzer, Sonja, Novak, Alexandra, Lohner, Karl and Zweytick, Dagmar (2011) In search of a novel target - Phosphatidylserine exposed by non-apoptotic tumor cells and metastases of malignancies with poor treatment efficacy. Biochimica et Biophysica Acta - Biomembranes, 1808 11: 2638-2645. doi:10.1016/j.bbamem.2011.07.026


Author Riedl, Sabrina
Rinner, Beate
Asslaber, Martin
Schaider, Helmut
Walzer, Sonja
Novak, Alexandra
Lohner, Karl
Zweytick, Dagmar
Title In search of a novel target - Phosphatidylserine exposed by non-apoptotic tumor cells and metastases of malignancies with poor treatment efficacy
Journal name Biochimica et Biophysica Acta - Biomembranes   Check publisher's open access policy
ISSN 0005-2736
Publication date 2011
Sub-type Article (original research)
DOI 10.1016/j.bbamem.2011.07.026
Open Access Status
Volume 1808
Issue 11
Start page 2638
End page 2645
Total pages 8
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract This study was performed in the aim to identify potential targets for the development of novel therapy to treat cancer with poor outcome or treatment efficacy. We show that the negatively charged phospholipid phosphatidylserine (PS) is exposed in the outer leaflet of their plasma membrane not only in tumor cell lines, but also in metastases and primary cultures thereof, which contrasts with a lack of PS exposure by differentiated non-tumorigenic counterparts. Studied tumor cell lines were derived from non-tumorigenic and malignant melanomas, prostate- and renal cancer, glioblastoma and a rhabdomyosarcoma. Importantly, also metastases of melanoma expose PS and there is a correlation between malignancy of melanoma cell lines from different stages of tumor progression and PS exposure. The PS exposure we found was neither of apoptotic nor of experimental artificial origin. Finally potentially malignant and non-malignant cells could be differentiated by sorting of a primary cell culture derived from a glioblastoma based on PS exposure, which has so far not been possible within one culture due to lack of a specific marker. Our data provide clear evidence that PS could serve as uniform marker of tumor cells and metastases as well as a target for novel therapeutic approaches based on e.g. PS-specific host defense derived peptides.
Keyword Cancer plasma membrane
Peptide target
Phosphatidylserine exposure
Tumor marker
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 63 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 62 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 01 May 2014, 09:52:40 EST by System User on behalf of School of Medicine