A petrol ether extract of the roots of Onosma paniculatum induces cell death in a caspase dependent manner

Rinner, Beate, Kretschmer, Nadine, Knausz, Heike, Mayer, Andrea, Boechzelt, Herbert, Hao, Xiao-Jiang, Heubl, Guenther, Efferth, Thomas, Schaider, Helmut and Bauer, Rudolf (2010) A petrol ether extract of the roots of Onosma paniculatum induces cell death in a caspase dependent manner. Journal of Ethnopharmacology, 129 2: 182-188. doi:10.1016/j.jep.2010.02.006


Author Rinner, Beate
Kretschmer, Nadine
Knausz, Heike
Mayer, Andrea
Boechzelt, Herbert
Hao, Xiao-Jiang
Heubl, Guenther
Efferth, Thomas
Schaider, Helmut
Bauer, Rudolf
Title A petrol ether extract of the roots of Onosma paniculatum induces cell death in a caspase dependent manner
Formatted title
A petrol ether extract of the roots of Onosma paniculatum induces cell death in a caspase dependent manner
Journal name Journal of Ethnopharmacology   Check publisher's open access policy
ISSN 0378-8741
1872-7573
Publication date 2010
Sub-type Article (original research)
DOI 10.1016/j.jep.2010.02.006
Open Access Status
Volume 129
Issue 2
Start page 182
End page 188
Total pages 7
Place of publication Shannon, Co. Clare, Ireland
Publisher Elsevier
Language eng
Formatted abstract
Aim of the study: Traditional Chinese medicine (TCM) has become very popular in Western countries during the last years. Zicao, a remedy of TCM, has been traditionally used to treat cancer, and, its main constituents, naphthoquinones, have been reported to possess antitumor activity (Chen et al., 2002; Papageorgiou et al., 1999). Here, we prepared extracts of different polarities of Onosma paniculatum Bur & Franch, a plant which is amongst others used as Zicao, but, much less investigated. The extracts were analyzed concerning their growth inhibitory and apoptosis-inducing activity in various tumor cells.

Materials and methods: Cell viability was measured by XTT viability and a growth inhibition assay. Effects on the cell cycle and caspase-3 were determined by flow cytometry.

Results: From three different extracts, a petrol ether extract showed significant growth inhibitory effect, cell cycle influence and caspase-3 dependent induction of apoptosis which was time and dose dependent.

Conclusion: To further determine the activity and mechanism of action of the petrol ether extract, we would like to isolate and identify the active principle and investigate the effects in more detail.
Keyword Cancer
Caspase-3
Cell cycle
Onosma paniculatum
Zicao
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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