Worldwide dissemination of acquired carbapenem-hydrolysing class D β-lactamases in Acinetobacter spp. other than Acinetobacter baumannii

Zander, Esther, Fernandez-Gonzalez, Ana, Schleicher, Xenia, Dammhayn, Cathrin, Kamolvit, Witchuda, Seifert, Harald and Higgins, Paul G. (2014) Worldwide dissemination of acquired carbapenem-hydrolysing class D β-lactamases in Acinetobacter spp. other than Acinetobacter baumannii. International Journal of Antimicrobial Agents, 43 4: 375-377. doi:10.1016/j.ijantimicag.2014.01.012


Author Zander, Esther
Fernandez-Gonzalez, Ana
Schleicher, Xenia
Dammhayn, Cathrin
Kamolvit, Witchuda
Seifert, Harald
Higgins, Paul G.
Title Worldwide dissemination of acquired carbapenem-hydrolysing class D β-lactamases in Acinetobacter spp. other than Acinetobacter baumannii
Formatted title
Worldwide dissemination of acquired carbapenem-hydrolysing class D β-lactamases in Acinetobacter spp. other than Acinetobacter baumannii
Journal name International Journal of Antimicrobial Agents   Check publisher's open access policy
ISSN 1872-7913
0924-8579
Publication date 2014-04
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.ijantimicag.2014.01.012
Open Access Status
Volume 43
Issue 4
Start page 375
End page 377
Total pages 3
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2015
Language eng
Formatted abstract
The aim of this study was to identify acquired OXA-type carbapenemases in Acinetobacter spp. other than Acinetobacter baumannii. From a total of 453 carbapenem-susceptible and -resistant Acinetobacter isolates collected worldwide, 23 were positive for blaOXA genes by multiplex PCR. These isolates were identified as Acinetobacter pittii (n = 18), Acinetobacter nosocomialis (n = 2),Acinetobacter junii (n = 1) and Acinetobacter genomic species 14TU/13BJ (n = 2). The blaOXA genes and associated insertion sequence (IS) elements were sequenced by primer walking. In 11 of these isolates, sequencing of the PCR products revealed that they were false-positive for blaOXA. The remaining 12 isolates, originating from Europe, Asia, South America, North America and South Africa, harboured OXA-23 (n = 4), OXA-58 (n = 5), OXA-40-like (n = 1) and OXA-143-like (n = 1); one A. pittii isolate harboured both OXA-23 and OXA-58. IS elements were associated with blaOXA in 10 isolates. OXA multiplex PCR showed a high degree of false-positive results (47.8%), indicating that detection of blaOXA in non-baumanniiAcinetobacter spp. should be confirmed using additional methods.
Keyword OXA multiplex PCR
Carbapenemase
Susceptibility
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2015 Collection
Official Audit
 
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