Wolbachia infection modifies the profile, shuttling and structure of MicroRNAs in a mosquito cell line

Mayoral, Jamie G., Etebari, Kayvan, Hussain, Mazhar, Khromykh, Alexander A. and Asgari, Sassan (2014) Wolbachia infection modifies the profile, shuttling and structure of MicroRNAs in a mosquito cell line. PLoS One, 9 4: . doi:10.1371/journal.pone.0096107


Author Mayoral, Jamie G.
Etebari, Kayvan
Hussain, Mazhar
Khromykh, Alexander A.
Asgari, Sassan
Title Wolbachia infection modifies the profile, shuttling and structure of MicroRNAs in a mosquito cell line
Formatted title
Wolbachia infection modifies the profile, shuttling and structure of MicroRNAs in a mosquito cell line
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-04-23
Year available 2014
Sub-type Article (original research)
DOI 10.1371/journal.pone.0096107
Open Access Status DOI
Volume 9
Issue 4
Total pages 16
Place of publication San Francisco, United States
Publisher Public Library of Science
Collection year 2015
Language eng
Formatted abstract
MicroRNAs (miRNAs) are small non-coding RNAs that play important roles in many biological processes such as development, cell signaling and immune response. Small RNA deep sequencing technology provided an opportunity for a thorough survey of the miRNA profile of a mosquito cell line from Aedes aegypti. We characterized the miRNA composition of the nucleus and the cytoplasm of uninfected cells and compared it with the one of cells infected with the endosymbiotic bacterium Wolbachia strain wMelPop-CLA. We found an overall increase of small RNAs between 18 and 28 nucleotides in both cellular compartments in Wolbachia-infected cells and identified specific miRNAs induced and/or suppressed by the Wolbachia infection. We discuss the mechanisms that the cell may use to shuttle miRNAs between the cytoplasm and the nucleus. In addition, we identified piRNAs that changed their abundance in response to Wolbachia infection. The miRNAs and piRNAs identified in this study provide promising leads for investigations into the host-endosymbiont interactions and for better understanding of how Wolbachia manipulates the host miRNA machinery in order to facilitate its persistent replication in infected cells.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article number e96107.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Biological Sciences Publications
School of Chemistry and Molecular Biosciences
 
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Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 17 times in Scopus Article | Citations
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Created: Thu, 24 Apr 2014, 09:51:43 EST by Prof Sassan Asgari on behalf of School of Biological Sciences