Fluorescent nucleic acid probes in living cells

Seefeld, Anne, Kokil, Ganesh R., Tupally, Karnaker R., Parekh, Harendra S. and Herten, Dirk-Peter (2013). Fluorescent nucleic acid probes in living cells. In Volker A. Erdmann and Jan Barciszewski (Ed.), DNA and RNA nanobiotechnologies in medicine: diagnosis and treatment of diseases (pp. 291-328) Berlin, Germany: Springer. doi:10.1007/978-3-642-36853-0_12


Author Seefeld, Anne
Kokil, Ganesh R.
Tupally, Karnaker R.
Parekh, Harendra S.
Herten, Dirk-Peter
Title of chapter Fluorescent nucleic acid probes in living cells
Title of book DNA and RNA nanobiotechnologies in medicine: diagnosis and treatment of diseases
Place of Publication Berlin, Germany
Publisher Springer
Publication Year 2013
Sub-type Research book chapter (original research)
DOI 10.1007/978-3-642-36853-0_12
Open Access Status
Series RNA Technologies
ISBN 9783642368523
9783642368530
Editor Volker A. Erdmann
Jan Barciszewski
Chapter number 12
Start page 291
End page 328
Total pages 38
Total chapters 17
Collection year 2014
Language eng
Abstract/Summary During the last decade a variety of fluorescent probes have been developed that allow quantitative analysis of specific DNA and RNA sequences in biological samples. In combination with recently developed methods in fluorescence microscopy, this offers the unique opportunity to quantitatively study DNA and RNA content in fixed and living cells in response to cellular stimulation. This chapter is intended to supply the reader with an overview of relevant approaches for probing specific DNA and RNA sequences in biological samples with a focus on DNA hairpin probes. We follow this with discussion on the modes of cellular entry from the perspective of both viral and non-viral carriers. To conclude we elaborate on the key barriers that need to be overcome for successful transfer of DNA probes into living cells.
Keyword Bioanalytical methods
Biosensors
Fluorescence
Nucleic acids (DNA, RNA)
Dendrimer
Gene delivery
Nonviral vectors
Q-Index Code BX
Q-Index Status Confirmed Code
Institutional Status UQ

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Created: Wed, 23 Apr 2014, 14:43:24 EST by Anthony Yeates on behalf of School of Pharmacy