SERS-barcoded colloidal gold NP assemblies as imaging agents for use in biodiagnostics

Dey, Priyanka, Olds, William, Blakey, Idriss, Thurecht, Kristofer J., Izake, Emad L. and Fredericks, Peter M. (2014). SERS-barcoded colloidal gold NP assemblies as imaging agents for use in biodiagnostics. In: Anita Mahadevan-Jansen and Wolfgang Petrich, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry. Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry, San Francisco, CA United States, (). 1-2 February 2014. doi:10.1117/12.2037159

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Author Dey, Priyanka
Olds, William
Blakey, Idriss
Thurecht, Kristofer J.
Izake, Emad L.
Fredericks, Peter M.
Title of paper SERS-barcoded colloidal gold NP assemblies as imaging agents for use in biodiagnostics
Conference name Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry
Conference location San Francisco, CA United States
Conference dates 1-2 February 2014
Proceedings title Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry
Journal name Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Series Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Place of Publication Bellingham, WA United States
Publisher SPIE
Publication Year 2014
Year available 2014
Sub-type Fully published paper
DOI 10.1117/12.2037159
Open Access Status
ISBN 9780819498526
ISSN 1605-7422
Editor Anita Mahadevan-Jansen
Wolfgang Petrich
Volume 8939
Total pages 10
Chapter number 9
Total chapters 39
Collection year 2015
Language eng
Abstract/Summary There is a growing need for new biodiagnostics that combine high throughput with enhanced spatial resolution and sensitivity. Gold nanoparticle (NP) assemblies with sub-10 nm particle spacing have the benefits of improving detection sensitivity via Surface enhanced Raman scattering (SERS) and being of potential use in biomedicine due to their colloidal stability. A promising and versatile approach to form solution-stable NP assemblies involves the use of multi-branched molecular linkers which allows tailoring of the assembly size, hot-spot density and interparticle distance. We have shown that linkers with multiple anchoring end-groups can be successfully employed as a linker to assemble gold NPs into dimers, linear NP chains and clustered NP assemblies. These NP assemblies with diameters of 30-120 nm are stable in solution and perform better as SERS substrates compared with single gold NPs, due to an increased hot-spot density. Thus, tailored gold NP assemblies are potential candidates for use as biomedical imaging agents. We observed that the hot-spot density and in-turn the SERS enhancement is a function of the linker polymer concentration and polymer architecture. New deep Raman techniques like Spatially Offset Raman Spectroscopy (SORS) have emerged that allow detection from beneath diffusely scattering opaque materials, including biological media such as animal tissue. We have been able to demonstrate that the gold NP assemblies could be detected from within both proteinaceous and high lipid containing animal tissue by employing a SORS technique with a backscattered geometry.
Keyword Deep tissue detection
Gold NP assemblies
Hyperbranched polymers
Imaging agents
Linker polymers
Q-Index Code E1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Tue, 22 Apr 2014, 02:04:14 EST by System User on behalf of Aust Institute for Bioengineering & Nanotechnology