Multiphoton fluorescence microscopy of the live kidney in health and disease

Small, David M., Sanchez, Washington Y., Roy, Sandrine, Hickey, Michael J. and Gobe, Glenda C. (2014) Multiphoton fluorescence microscopy of the live kidney in health and disease. Journal of Biomedical Optics, 19 2: . doi:10.1117/1.JBO.19.2.020901


Author Small, David M.
Sanchez, Washington Y.
Roy, Sandrine
Hickey, Michael J.
Gobe, Glenda C.
Title Multiphoton fluorescence microscopy of the live kidney in health and disease
Journal name Journal of Biomedical Optics   Check publisher's open access policy
ISSN 1083-3668
1560-2281
Publication date 2014-02
Year available 2014
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1117/1.JBO.19.2.020901
Open Access Status DOI
Volume 19
Issue 2
Total pages 13
Place of publication Bellingham, WA, United States
Publisher S P I E - International Society for Optical Engineering
Collection year 2015
Language eng
Formatted abstract
The structural and functional heterogeneity of the kidney ensures a diversity of response in health and disease. Multiphoton microscopy has improved our understanding of kidney physiology and pathophysiology by enabling the visualization of the living kidney in comparison with the static view of previous technologies. The use of multiphoton microscopy with rodent models in conjunction with endogenous fluorescence and exogenous infused dyes permits the measurement of renal processes, such as glomerular permeability, juxtaglomerular apparatus function, tubulointerstitial function, tubulovascular interactions, vascular flow rate, and the intrarenal renin-angiotensin-aldosterone system. Subcellular processes, including mitochondrial dynamics, reactive oxygen species production, cytosolic ion concentrations, and death processes apoptosis and necrosis, can also be measured by multiphoton microscopy. This has allowed valuable insight into the pathophysiology of diabetic nephropathy, renal ischemia-reperfusion injury, hypertensive nephropathy, as well as inflammatory responses of the kidney. The current review presents an overview of multiphoton microscopy with a focus on techniques for imaging the kidney and gives examples of instances where multiphoton microscopy has been utilized to study renal pathophysiology in the living kidney. With continued advancements in the field of biological optics and increased adoption in experimental nephrology, multiphoton microscopy will undoubtedly continue to create new paradigms in kidney disease.
Keyword Kidney
Multiphoton microscopy
Fluorescence lifetime imaging
Pathophysiology
Basal Cell Carcinoma
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Official 2015 Collection
School of Medicine Publications
UQ Diamantina Institute Publications
 
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