Nucleic acid extraction from biomining microorganisms

Zammit, C. M., Mutch, L. A., Watling, H. R. and Watkin, E. L. J. (2009). Nucleic acid extraction from biomining microorganisms. In: Edgardo R. Donati, Marisa R. Viera, Eduardo L. Tavani, María A. Giaveno, Teresa L. Lavalle and Patricia A. Chiacchiarini, Biohydrometallurgy: a Meeting Point Between Microbial Ecology, Metal Recovery Processes and Environmental Remediation. 18th International Biohydrometallurgy Symposium, Bariloche, Argentina, (159-162). 13-17 September 2009. doi:10.4028/

Author Zammit, C. M.
Mutch, L. A.
Watling, H. R.
Watkin, E. L. J.
Title of paper Nucleic acid extraction from biomining microorganisms
Conference name 18th International Biohydrometallurgy Symposium
Conference location Bariloche, Argentina
Conference dates 13-17 September 2009
Proceedings title Biohydrometallurgy: a Meeting Point Between Microbial Ecology, Metal Recovery Processes and Environmental Remediation   Check publisher's open access policy
Journal name Advanced Materials Research   Check publisher's open access policy
Place of Publication Zurich, Switzerland
Publisher Trans Tech Publications
Publication Year 2009
Sub-type Fully published paper
DOI 10.4028/
Open Access Status
ISBN 9780878493227
ISSN 1022-6680
Editor Edgardo R. Donati
Marisa R. Viera
Eduardo L. Tavani
María A. Giaveno
Teresa L. Lavalle
Patricia A. Chiacchiarini
Volume 71-73
Start page 159
End page 162
Total pages 4
Language eng
Abstract/Summary Various methods of nucleic acid (NA) extraction were investigated with the aim of developing a quantitative method of NA extraction from five representative strains of biomining microorganisms. The process of removing cells from mineral surfaces, lysing microorganisms, precipitating NA and purifying RNA were analysed. The success of each method was examined spectrophotometrically, by agarose gel electrophoresis and PCR or quantitative real time PCR (qPCR). The most important step was shown to be cellular lysis, which principally impacted on the quantity of NA extracted from each strain. The quantity and quality of extracted NA was highly dependent on the method of NA precipitation. This study resulted in the development of a NA extraction method that reliably and reproducibly extracted NA from five strains of biomining microorganisms.
Keyword DNA extraction
RNA extraction
Molecular methods
Mine drainage
Q-Index Code E1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Conference Paper
Collection: School of Earth Sciences Publications
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