F9 fimbriae of uropathogenic Escherichia coli are expressed at low temperature and recognise Galβ1-3GlcNAc-containing glycans

Wurpel, Daniël J., Totsika, Makrina, Allsopp, Luke P., Hartley-Tassell, Lauren E., Day, Christopher J., Peters, Kate M., Sarkar, Sohinee, Ulett, Glen C., Yang, Ji, Tiralongo, Joe, Strugnell, Richard A., Jennings, Michael P. and Schembri, Mark A. (2014) F9 fimbriae of uropathogenic Escherichia coli are expressed at low temperature and recognise Galβ1-3GlcNAc-containing glycans. PLoS One, 9 3: . doi:10.1371/journal.pone.0093177


Author Wurpel, Daniël J.
Totsika, Makrina
Allsopp, Luke P.
Hartley-Tassell, Lauren E.
Day, Christopher J.
Peters, Kate M.
Sarkar, Sohinee
Ulett, Glen C.
Yang, Ji
Tiralongo, Joe
Strugnell, Richard A.
Jennings, Michael P.
Schembri, Mark A.
Title F9 fimbriae of uropathogenic Escherichia coli are expressed at low temperature and recognise Galβ1-3GlcNAc-containing glycans
Formatted title
F9 fimbriae of uropathogenic Escherichia coli are expressed at low temperature and recognise Galβ1-3GlcNAc-containing glycans

Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-03-26
Year available 2014
Sub-type Article (original research)
DOI 10.1371/journal.pone.0093177
Open Access Status DOI
Volume 9
Issue 3
Total pages 13
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2015
Language eng
Formatted abstract
Uropathogenic Escherichia coli (UPEC) is the leading causative agent of urinary tract infections (UTI) in the developed world. Among the major virulence factors of UPEC, surface expressed adhesins mediate attachment and tissue tropism. UPEC strains typically possess a range of adhesins, with type 1 fimbriae and P fimbriae of the chaperone-usher class the best characterised. We previously identified and characterised F9 as a new chaperone-usher fimbrial type that mediates biofilm formation. However, the regulation and specific role of F9 fimbriae remained to be determined in the context of wild-type clinical UPEC strains. In this study we have assessed the distribution and genetic context of the f9 operon among diverse E. coli lineages and pathotypes and demonstrated that f9 genes are significantly more conserved in a UPEC strain collection in comparison to the well-defined E. coli reference (ECOR) collection. In the prototypic UPEC strain CFT073, the global regulator protein H-NS was identified as a transcriptional repressor of f9 gene expression at 37°C through its ability to bind directly to the f9 promoter region. F9 fimbriae expression was demonstrated at 20°C, representing the first evidence of functional F9 fimbriae expression by wild-type E. coli. Finally, glycan array analysis demonstrated that F9 fimbriae recognise and bind to terminal Galβ1-3GlcNAc structures.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Wed, 09 Apr 2014, 11:18:57 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences