Variation in the hindgut microbial communities of the Florida manatee, Trichechus manatus latirostris over winter in Crystal River, Florida

Merson, Samuel D., Ouwerkerk, Diane, Gulino, Lisa-Maree, Klieve, Athol, Bonde, Robert K., Burgess, Elizabeth A. and Lanyon, Janet M. (2014) Variation in the hindgut microbial communities of the Florida manatee, Trichechus manatus latirostris over winter in Crystal River, Florida. FEMS Microbiology Ecology, 87 3: 601-615. doi:10.1111/1574-6941.12248


Author Merson, Samuel D.
Ouwerkerk, Diane
Gulino, Lisa-Maree
Klieve, Athol
Bonde, Robert K.
Burgess, Elizabeth A.
Lanyon, Janet M.
Title Variation in the hindgut microbial communities of the Florida manatee, Trichechus manatus latirostris over winter in Crystal River, Florida
Formatted title
Variation in the hindgut microbial communities of the Florida manatee, Trichechus manatus latirostris over winter in Crystal River, Florida
Journal name FEMS Microbiology Ecology   Check publisher's open access policy
ISSN 0168-6496
1574-6941
Publication date 2014-03
Year available 2013
Sub-type Article (original research)
DOI 10.1111/1574-6941.12248
Open Access Status
Volume 87
Issue 3
Start page 601
End page 615
Total pages 15
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell
Collection year 2014
Language eng
Formatted abstract
The Florida manatee, Trichechus manatus latirostris, is a hindgut-fermenting herbivore. In winter, manatees migrate to warm water overwintering sites where they undergo dietary shifts and may suffer from cold-induced stress. Given these seasonally induced changes in diet, the present study aimed to examine variation in the hindgut bacterial communities of wild manatees overwintering at Crystal River, west Florida. Faeces were sampled from 36 manatees of known sex and body size in early winter when manatees were newly arrived and then in mid-winter and late winter when diet had probably changed and environmental stress may have increased. Concentrations of faecal cortisol metabolite, an indicator of a stress response, were measured by enzyme immunoassay. Using 454-pyrosequencing, 2027 bacterial operational taxonomic units were identified in manatee faeces following amplicon pyrosequencing of the 16S rRNA gene V3/V4 region. Classified sequences were assigned to eight previously described bacterial phyla; only 0.36% of sequences could not be classified to phylum level. Five core phyla were identified in all samples. The majority (96.8%) of sequences were classified as Firmicutes (77.3 ± 11.1% of total sequences) or Bacteroidetes (19.5 ± 10.6%). Alpha-diversity measures trended towards higher diversity of hindgut microbiota in manatees in mid-winter compared to early and late winter. Beta-diversity measures, analysed through permanova, also indicated significant differences in bacterial communities based on the season.
Keyword 16S rRNA gene
454-pyrosequencing
Bacteria
Colon
Faeces
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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