Lovastatin downregulates renal myofibroblast function in vitro

Kelynack, Kristen J., Hewitson, Tim D., Martic, Marina, Mctaggart, Steven and Becker, Gavin J. (2002) Lovastatin downregulates renal myofibroblast function in vitro. Nephron, 91 4: 701-707. doi:10.1159/000065034


Author Kelynack, Kristen J.
Hewitson, Tim D.
Martic, Marina
Mctaggart, Steven
Becker, Gavin J.
Title Lovastatin downregulates renal myofibroblast function in vitro
Journal name Nephron   Check publisher's open access policy
ISSN 0028-2766
2235-3186
Publication date 2002-08-01
Sub-type Article (original research)
DOI 10.1159/000065034
Open Access Status Not yet assessed
Volume 91
Issue 4
Start page 701
End page 707
Total pages 7
Place of publication Basel, Switzerland
Publisher S. Karger AG
Language eng
Formatted abstract
Interstitial fibrosis is recognised as the best histological predictor of progressive renal disease. Myofibroblasts contribute to this process through several functions including hyperproliferation, collagen and collagenase synthesis and reorganisation of extracellular matrix. Recent limited in vitro studies suggest that 3-hydroxy-3-methylglutaryl-coenzyme A (HMG CoA) reductase inhibitors may reduce renal injury not only through their lipid-lowering effects but also by antagonising myofibroblast function. This study therefore examined the effects of lovastatin on the above interstitial myofibroblast behaviours in vitro. Primary cultures of rat renal cortical myofibroblasts were grown by explantation and characterised by immunohistochemistry. Dose response effects of lovastatin (0, 15, 30 μM) in DMEM and 10% FCS were examined on myofibroblast kinetics, total collagen synthesis, collagen I lattice contraction and actin filament rearrangement. Lovastatin decreased myofibroblast proliferation and growth. Likewise, collagen I lattice contraction and actin filament rearrangement were partially inhibited when lovastatin was added at 30 μM. In addition, lovastatin decreased both collagen and collagenase synthesis. Our results suggest that myofibroblast function may be downregulated by lovastatin in vitro. Although a decrease in myofibroblast activity may offer potential benefit in the prevention of progressive scarring, further studies will be necessary to determine the relative importance of these functions. 
Keyword Collagen
Fibroblast
HMG-CoA reductase inhibitor
Renal
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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