Enzymatic antibody tagging: toward a universal biocompatible targeting tool

Ta, Hang T., Peter, Karlheinz and Hagemeyer, Christoph E. (2012) Enzymatic antibody tagging: toward a universal biocompatible targeting tool. Trends in Cardiovascular Medicine, 22 4: 105-111. doi:10.1016/j.tcm.2012.07.004


Author Ta, Hang T.
Peter, Karlheinz
Hagemeyer, Christoph E.
Title Enzymatic antibody tagging: toward a universal biocompatible targeting tool
Journal name Trends in Cardiovascular Medicine   Check publisher's open access policy
ISSN 1050-1738
1873-2615
Publication date 2012-05
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1016/j.tcm.2012.07.004
Open Access Status
Volume 22
Issue 4
Start page 105
End page 111
Total pages 7
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Language eng
Abstract Targeted delivery or “smart delivery” of pharmaceutical or imaging agents and even entire cells such as stem cells is an emerging trend in modern biotechnology. A binding ligand such as an antibody that can specifically bind to receptors expressed at a disease site is an essential component of such constructs. Different chemical methods have been widely used to apply antibodies for delivery systems; however, they typically result in impairment or loss of antibody functionality. Enzyme-mediated conjugation approaches have been developed to overcome this major disadvantage of conventional chemical methods. Sortase, an enzyme derived from Staphylococcus aureus, is able to provide a biochemically robust, highly reproducible, and site-specific coupling method for the conjugation of antibodies to pharmaceutical agents, nanoparticles, and cells for drug delivery, molecular imaging, and cell homing. Here, we review the use of sortase and other enzyme-based methods as bioconjugation tools with a focus on cardiovascular applications.
Keyword Single-chain antibody
Conformation-specific blockade
Mediated protein ligation
Biotin ligase
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 10 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 04 Apr 2014, 18:48:58 EST by System User on behalf of Aust Institute for Bioengineering & Nanotechnology