Global dissemination of a multidrug resistant Escherichia coli clone

Petty, Nicola K., Ben Zakour, Nouri L., Stanton-Cook, Mitchell, Skippington, Elizabeth, Totsika, Makrina, Forde, Brian M., Phan, Minh-Duy, Gomes Moriel, Danilo, Peters, Kate M., Davies, Mark, Rogers, Benjamin A., Dougan, Gordon, Rodriguez-Baño, Jesús, Pascual, Alvaro, Pitout, Johann D., Upton, Mathew, Paterson, David L., Walsh, Timothy R., Schembri, Mark A. and Beatson, Scott A. (2014) Global dissemination of a multidrug resistant Escherichia coli clone. Proceedings of the National Academy of Sciences, 111 15: 5694-5699. doi:10.1073/pnas.1322678111


Author Petty, Nicola K.
Ben Zakour, Nouri L.
Stanton-Cook, Mitchell
Skippington, Elizabeth
Totsika, Makrina
Forde, Brian M.
Phan, Minh-Duy
Gomes Moriel, Danilo
Peters, Kate M.
Davies, Mark
Rogers, Benjamin A.
Dougan, Gordon
Rodriguez-Baño, Jesús
Pascual, Alvaro
Pitout, Johann D.
Upton, Mathew
Paterson, David L.
Walsh, Timothy R.
Schembri, Mark A.
Beatson, Scott A.
Title Global dissemination of a multidrug resistant Escherichia coli clone
Formatted title
Global dissemination of a multidrug resistant Escherichia coli clone
Journal name Proceedings of the National Academy of Sciences   Check publisher's open access policy
ISSN 0027-8424
1091-6490
Publication date 2014-04-15
Sub-type Article (original research)
DOI 10.1073/pnas.1322678111
Open Access Status DOI
Volume 111
Issue 15
Start page 5694
End page 5699
Total pages 6
Editor Scott J. Hultgren
Place of publication Washington, DC, United States
Publisher National Academy of Sciences
Collection year 2015
Language eng
Formatted abstract
Escherichia coli sequence type 131 (ST131) is a globally disseminated, multidrug resistant (MDR) clone responsible for a high proportion of urinary tract and bloodstream infections. The rapid emergence and successful spread of E. coli ST131 is strongly associated with several factors, including resistance to fluoroquinolones, high virulence gene content, the possession of the type 1 fimbriae FimH30 allele, and the production of the CTX-M-15 extended spectrum β-lactamase (ESBL). Here, we used genome sequencing to examine the molecular epidemiology of a collection of E. coli ST131 strains isolated from six distinct geographical locations across the world spanning 2000–2011. The global phylogeny of E. coli ST131, determined from whole-genome sequence data, revealed a single lineage of E. coli ST131 distinct from other extraintestinal E. coli strains within the B2 phylogroup. Three closely related E. coli ST131 sublineages were identified, with little association to geographic origin. The majority of single-nucleotide variants associated with each of the sublineages were due to recombination in regions adjacent to mobile genetic elements (MGEs). The most prevalent sublineage of ST131 strains was characterized by fluoroquinolone resistance, and a distinct virulence factor and MGE profile. Four different variants of the CTX-M ESBL–resistance gene were identified in our ST131 strains, with acquisition of CTX-M-15 representing a defining feature of a discrete but geographically dispersed ST131 sublineage. This study confirms the global dispersal of a single E. coli ST131 clone and demonstrates the role of MGEs and recombination in the evolution of this important MDR pathogen.

Significance
Escherichia coli sequence type 131 (ST131) is a globally disseminated multidrug-resistant clone associated with human urinary tract and bloodstream infections. Here, we have used genome sequencing to map the temporal and spatial relationship of a large collection of E. coli ST131 strains isolated from six distinct geographical regions across the world. We show that E. coli ST131 strains are distinct from other extraintestinal pathogenic E. coli and arose from a single progenitor strain prior to the year 2000.
Keyword Bacterial evolution
Genomics
Phylogeography
Genomic epidemiology
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Wed, 02 Apr 2014, 16:18:54 EST by Nouri Ben Zakour on behalf of School of Chemistry & Molecular Biosciences