Cytotoxic evaluation of four Haplophyllum species with various tumor cell lines

Varamini, P., Doroudchi, M., Mohagheghzadeh, A., Soltani, M. and Ghaderi, A. (2007) Cytotoxic evaluation of four Haplophyllum species with various tumor cell lines. Pharmaceutical Biology, 45 4: 299-302. doi:10.1080/13880200701214938

Author Varamini, P.
Doroudchi, M.
Mohagheghzadeh, A.
Soltani, M.
Ghaderi, A.
Title Cytotoxic evaluation of four Haplophyllum species with various tumor cell lines
Formatted title
Cytotoxic evaluation of four Haplophyllum species with various tumor cell lines
Journal name Pharmaceutical Biology   Check publisher's open access policy
ISSN 1388-0209
Publication date 2007
Sub-type Article (original research)
DOI 10.1080/13880200701214938
Open Access Status
Volume 45
Issue 4
Start page 299
End page 302
Total pages 4
Place of publication London, United Kingdom
Publisher Informa Healthcare
Language eng
Formatted abstract
Total extracts of the aerial parts of four Haplophyllum. species (Rutaceae) including Haplophyllum stapfianum. Hand.-Mazz., H. tuberculatum. (Forssk.) Adr. Juss., H. acutifolium. (DC.) G. Don, and H. viridulum. Soják were tested for in vitro. cytotoxicity against 11 tumor cell lines and normal peripheral blood mononuclear cells (PBMCs). Cytotoxicity of Haplophyllum. species were also compared with Ruta graveolens. L. (Rutaceae) extract. Cytotoxic activity of extracts was evaluated by the WST-1 assay and expressed as IC50 values. The results obtained indicated that among hematopoietic tumor cell lines, strong cytotoxic activity was observed from H. stapfianum., H. tuberculatum., and H. acutifolium. against RAMOS cell line with IC50 values of 12.3, 25.3, and 23.7 µg/mL, respectively; H. stapfianum. and H. tuberculatum. against U937 cell line with IC50 values of 15.6 and 29.3 µg/mL; and H. tuberculatum. against RPMI-8866 cell line with IC50 = 31.8 µg/mL. Testing plant extracts on solid tumor cell lines revealed that all Haplophyllum. species have markedly high cytotoxicity against LNCap-FGC-10, a prostate adenocarcinoma cell line, except H. viridulum.. H. tuberculatum. also showed strong cytotoxic effect against 5637, a bladder carcinoma cell line (IC50 = 23.3 µg/mL). Investigating cytotoxic activity of R. graveolens. resulted in higher IC50 values against all tumor cell lines except LNCap-FGC-10 (IC50 = 27.6 µg/mL). Cytotoxicity evaluation of Haplophyllum. species on PBMCs revealed that resulting IC50 values for H. stapfianum. and H. acutifolium. on tumor cell lines with high cytotoxic activity were significantly lower than the IC50 values obtained for PBMCs (p < 0.05).
Keyword Cell line
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
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Created: Mon, 31 Mar 2014, 14:42:24 EST by Dr Pegah Varamini on behalf of School of Chemistry & Molecular Biosciences