Cell cycle analysis and cytotoxic potential of Ruta graveolens against human tumor cell lines

Varamini, P., Soltani, M. and Ghaderi, A. (2009) Cell cycle analysis and cytotoxic potential of Ruta graveolens against human tumor cell lines. Neoplasma, 56 6: 490-493. doi:10.4149/neo_2009_06_490


Author Varamini, P.
Soltani, M.
Ghaderi, A.
Title Cell cycle analysis and cytotoxic potential of Ruta graveolens against human tumor cell lines
Journal name Neoplasma   Check publisher's open access policy
ISSN 0028-2685
1338-4317
Publication date 2009
Sub-type Article (original research)
DOI 10.4149/neo_2009_06_490
Open Access Status
Volume 56
Issue 6
Start page 490
End page 493
Total pages 4
Place of publication Bratislava, Slovakia
Publisher AEPress, s.r.o.
Language eng
Abstract There are reports on the presence of various compounds exerting different biological activities in Ruta graveolens, a plant of Rutaceae family. The aim of the present study was to evaluate in vitro cytotoxicity of the total extract of R. graveolens against tumor cell lines of different origin. Aerial parts of the plant was extracted with 70% ethanol by sonication method and cytotoxic activity was examined on RAJI, RAMOS, RPMI8866, U937, Jurkat, MDA-MB-453, MCF-7, LNCap-FGC-10, 5637, HeLa, SK-OV-3, A549, Mehr-80 and also peripheral blood mononuclear cells (PBMC) by the use of WST-1 assay. Results were expressed as IC50 values. R. graveolens extract showed high cytotoxic activity against RAJI and RAMOS, two Burkitt’s lymphoma cell lines, with an IC50 equal to 24.3µg/ml and 35.2µg/ml respectively and LNCap-FGC-10, a prostate adenocarcinoma cell line with an IC50 equal to 27.6µg/ml as well as Mehr-80, a newly established Large Cell Lung Carcinoma (IC50=46.2 µg/ml). No significant anti-proliferative activity was observed on other cell lines including MCF-7, MDA-MB-453, SK-OV-3, HeLa, 5637, JURKAT and RPMI8866. Adverse cytotoxic effect of R. graveolens was investigated against PBMCs and a significantly lower effect of this extract (IC50=104 µg/ml) was seen on normal cells compared with RAJI and RAMOS, two haematopoietic cell lines.
Keyword Ruta graveolens
Rutaceae
Cell line
Cytotoxicity
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Mon, 31 Mar 2014, 14:04:33 EST by Dr Pegah Varamini on behalf of School of Chemistry & Molecular Biosciences