Early gene expression programs accompanying trans-differentiation of epidermal cells of Vicia faba cotyledons into transfer cells

Dibley, Stphen J., Zhou, Yuchan, Andriunas, Felicity A., Talbot, Mark J., Offler, Christina E., Patrick, John W. and McCurdy, David W. (2009) Early gene expression programs accompanying trans-differentiation of epidermal cells of Vicia faba cotyledons into transfer cells. New Phytologist, 182 4: 863-877. doi:10.1111/j.1469-8137.2009.02822.x


Author Dibley, Stphen J.
Zhou, Yuchan
Andriunas, Felicity A.
Talbot, Mark J.
Offler, Christina E.
Patrick, John W.
McCurdy, David W.
Title Early gene expression programs accompanying trans-differentiation of epidermal cells of Vicia faba cotyledons into transfer cells
Journal name New Phytologist   Check publisher's open access policy
ISSN 0028-646X
1469-8137
Publication date 2009
Year available 2009
Sub-type Article (original research)
DOI 10.1111/j.1469-8137.2009.02822.x
Open Access Status
Volume 182
Issue 4
Start page 863
End page 877
Total pages 15
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing Ltd.
Collection year 2010
Language eng
Subject 1110 Nursing
1314 Physiology
Abstract Transfer cells (TCs) trans-differentiate from differentiated cells by developing extensive wall ingrowths that enhance plasma membrane transport of nutrients. Here, we investigated transcriptional changes accompanying induction of TC development in adaxial epidermal cells of cultured Vicia faba cotyledons. • Global changes in gene expression revealed by cDNA-AFLP were compared between adaxial epidermal cells during induction (3 h) and subsequent building (24 h) of wall ingrowths, and in cells of adjoining storage parenchyma tissue, which do not form wall ingrowths. • A total of 5795 transcript-derived fragments (TDFs) were detected; of these, 264 TDFs showed epidermal-specific changes in gene expression and a further 207 TDFs were differentially expressed in both epidermal and storage parenchyma cells. Genes involved in signalling (auxin/ethylene), metabolism (mitochondrial; storage product hydrolysis), cell division, vesicle trafficking and cell wall biosynthesis were specifically induced in epidermal TCs. Blockers of auxin action and vesicle trafficking inhibited ingrowth formation and marked increases in cell division accompanied TC development. • Auxin and possibly ethylene signalling cascades induce epidermal cells of V. faba cotyledons to trans-differentiate into TCs. Trans-differentiation is initiated by rapid de-differentiation to a mitotic state accompanied by mitochondrial biogenesis driving storage product hydrolysis to fuel wall ingrowth formation orchestrated by a modified vesicle trafficking mechanism.
Keyword Trans-differentiation
Transfer cells
Vicia faba
Wall ingrowths
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 23 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 25 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 21 Mar 2014, 14:54:40 EST by Yuchan Zhou on behalf of Qld Alliance for Agriculture and Food Innovation