Tonoplast lipid composition and proton pump of pineapple fruit during low-temperature storage and blackheart development

Zhou, Yuchan, Pan, Xiaoping, Qu, Hongxia and Underhill, Steven J. R. (2014) Tonoplast lipid composition and proton pump of pineapple fruit during low-temperature storage and blackheart development. Journal of Membrane Biology, 247 5: 429-439. doi:10.1007/s00232-014-9650-3


Author Zhou, Yuchan
Pan, Xiaoping
Qu, Hongxia
Underhill, Steven J. R.
Title Tonoplast lipid composition and proton pump of pineapple fruit during low-temperature storage and blackheart development
Journal name Journal of Membrane Biology   Check publisher's open access policy
ISSN 0022-2631
1432-1424
Publication date 2014-03-22
Sub-type Article (original research)
DOI 10.1007/s00232-014-9650-3
Volume 247
Issue 5
Start page 429
End page 439
Total pages 11
Place of publication New York, NY, United States
Publisher Springer
Collection year 2015
Language eng
Abstract Vacuole represents a major storage organelle playing vital roles in pH homoeostasis and cellular detoxification. The chemical and functional properties of tonoplast in response to chilling temperature and their roles in chilling injury are largely unknown. In the current study, lipid composition of tonoplast and the activities of two vacuolar proton pumps, H+-ATPase (V-ATPase) and H+-pyrophosphatase (V-PPase), were investigated in accordance with the development of blackheart, a form of chilling injury in pineapple fruit (Ananas comosus). Chilling temperature at 10 °C for 1 week induced irreversible blackheart injury in concurrence with a substantial decrease in V-ATPase activity. By contrast, the activity was increased after 1 week at 25 °C. The activity of V-PPase was not changed under both temperatures. Level of total phospholipids of tonoplast decreased at 10 °C, but increased at 25 °C. There was no change at the level of total glycolipids under both temperatures. Thus, low temperature increased the ratio of total glycolipids vs. total phospholipids of tonoplast. Phosphatidylcholine and phosphatidylethanolamine were the predominant phospholipids of tonoplast. Low temperature increased the relative level of phosphatidic acid but decreased the percentage of both phosphatidylcholine and phosphatidylethanolamine. Unsaturated fatty acids accounted for over 60 % of the total tonoplast fatty acids, with C18:1 and C18:2 being predominant. Low temperature significantly decreased the percentage of C18:3. Modification of membrane lipid composition and its effect on the functional property of tonoplast at low temperature were discussed in correlation with their roles in the development of chilling injury in pineapple fruit.
Keyword Tonoplast
Vacuolar H?-ATPase
Vacuolar H?-pyrophosphatase
Lipid composition
Chilling injury
Pineapple fruit
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2015 Collection
 
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Created: Sat, 22 Mar 2014, 00:32:33 EST by Yuchan Zhou on behalf of Qld Alliance for Agriculture and Food Innovation