Double stranded RNA expression and its topical application for non-transgenic resistance to plant viruses

Robinson, Karl E., Worrall, Elizabeth A. and Mitter, Neena (2014) Double stranded RNA expression and its topical application for non-transgenic resistance to plant viruses. Journal of Plant Biochemistry and Biotechnology, Online First 3: 1-7. doi:10.1007/s13562-014-0260-z

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Author Robinson, Karl E.
Worrall, Elizabeth A.
Mitter, Neena
Title Double stranded RNA expression and its topical application for non-transgenic resistance to plant viruses
Journal name Journal of Plant Biochemistry and Biotechnology   Check publisher's open access policy
ISSN 0971-7811
Publication date 2014-03-06
Year available 2014
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1007/s13562-014-0260-z
Volume Online First
Issue 3
Start page 1
End page 7
Total pages 7
Place of publication Gandharva Mahavidyalaya, New Delhi, India
Publisher Springer (India) Private
Collection year 2015
Language eng
Formatted abstract
The discovery of RNA silencing has heralded a new phase in the understanding of nucleic acid metabolism and has provided researchers with alternative methods to define the biochemical, developmental and self-protection pathways in plants. Manipulation of these pathways to ultimately modify, induce or enhance favorable traits and repress or completely eliminate unfavorable qualities including disease susceptibility can be achieved by insertion or infiltration of transgenes encoding appropriate effectors. Remarkably, in contrast to transgene derived viral resistance, the RNA silencing mechanism can be readily induced by the topical application of dsRNA effector molecules. The current review summarises the various dsRNA production systems available for producing the large amounts of dsRNA required for topical application onto field grown crops. Also discussed are several investigations utilising a topical application of exogenous, sequence-specific dsRNA to induce the RNA silencing mechanism for viral resistance in plants. Currently, there are limitations for the spray-on application of dsRNA for field use. Further research to define a new phase in the non-transgenic delivery of RNA silencing for complete management of plant pathogens is crucial for its commercial application.
Keyword RNA silencing
dsRNA
Topical application
Non transgenic
Virus resistance
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online: 06 March 2014

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2015 Collection
 
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Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 4 times in Scopus Article | Citations
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Created: Fri, 21 Mar 2014, 23:29:59 EST by Dr Neena Mitter on behalf of Centre for Plant Science