Obesity and sex influence insulin resistance and total and multimer adiponectin levels in adult neutered domestic shorthair client-owned cats

Bjornvad, C. R., Rand, J. S., Tan, H. Y., Jensen, K. S., Rose, F. J., Armstrong, P. J. and Whitehead, J. P. (2014) Obesity and sex influence insulin resistance and total and multimer adiponectin levels in adult neutered domestic shorthair client-owned cats. Domestic Animal Endocrinology, 47 1: 55-64. doi:10.1016/j.domaniend.2013.11.006


Author Bjornvad, C. R.
Rand, J. S.
Tan, H. Y.
Jensen, K. S.
Rose, F. J.
Armstrong, P. J.
Whitehead, J. P.
Title Obesity and sex influence insulin resistance and total and multimer adiponectin levels in adult neutered domestic shorthair client-owned cats
Journal name Domestic Animal Endocrinology   Check publisher's open access policy
ISSN 0739-7240
1879-0054
Publication date 2014-04-01
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.domaniend.2013.11.006
Volume 47
Issue 1
Start page 55
End page 64
Total pages 10
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Collection year 2014
Language eng
Formatted abstract
In this study, we estimated insulin sensitivity and determined plasma concentrations of total-, low-molecular-weight (LMW), and high-molecular-weight (HMW) adiponectin and leptin in 72 domestic shorthair, neutered, client-owned cats. Glucose tolerance was assessed with an intravenous glucose tolerance test and body fat percentage (BF%) was measured with dual-energy x-ray absorptiometry. Total adiponectin was measured with 2 different ELISAs. Low-molecular-weight and HMW adiponectin plasma concentrations were determined by Western blot analysis after sucrose-gradient velocity centrifugation, and the adiponectin multimer ratio [SA = HMW/(HMW + LMW)] was calculated. Differences in glucose tolerance, leptin, total adiponectin, and multimer ratio among lean (BF% <35; n = 26), overweight (35 <BF% <45; n = 28), and obese (BF% >45; n = 18) cats as well as between male (n = 34) and female (n = 38) neutered cats were evaluated by linear regression and 2-way ANOVA. Sex and age were included as covariates for analysis of BF%, whereas BF%, fat mass, and lean body mass were covariates for analysis of sex differences. Increased BF% was negatively correlated with multimer ratio (SA, r = −45; P < 0.002), whereas no differences were found in total adiponectin concentrations among BF% groups (P > 0.01). Male cats had indices of decreased insulin tolerance and significantly lower total adiponectin concentrations than did female cats (mean ± SEM, 3.7 ± 0.4 vs 5.4 ± 0.5 μg/mL; P < 0.02). Altered SAs could contribute to an obesity-associated decreasing glucose tolerance in cats, and low total adiponectin concentrations may relate to increased risk of diabetes mellitus in neutered male cats.
Keyword Adipokine
Feline
Gender
Dual-energy x-ray absorptiometry
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 5 December 2013

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Veterinary Science Publications
UQ Diamantina Institute Publications
 
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Created: Thu, 20 Mar 2014, 22:07:06 EST by Kylie Hengst on behalf of Centre for Companion Animal Health