Genes regulating molecular and cellular functions in noninfectious nonallergic rhinitis

Cardell, L. O., Andersson, M., Cervin, A., Davidsson, A., Hellgren, J., Holmstrom, M., Lundblad, L., Stierna, P., Stjarne, P., Adner, M. and SRFA study group (2009) Genes regulating molecular and cellular functions in noninfectious nonallergic rhinitis. Allergy: European Journal of Allergy and Clinical Immunology, 64 9: 1301-1308. doi:10.1111/j.1398-9995.2009.02009.x


Author Cardell, L. O.
Andersson, M.
Cervin, A.
Davidsson, A.
Hellgren, J.
Holmstrom, M.
Lundblad, L.
Stierna, P.
Stjarne, P.
Adner, M.
SRFA study group
Title Genes regulating molecular and cellular functions in noninfectious nonallergic rhinitis
Journal name Allergy: European Journal of Allergy and Clinical Immunology   Check publisher's open access policy
ISSN 0105-4538
1398-9995
Publication date 2009
Sub-type Article (original research)
DOI 10.1111/j.1398-9995.2009.02009.x
Open Access Status
Volume 64
Issue 9
Start page 1301
End page 1308
Total pages 8
Place of publication Chichester, West Sussex, United Kingdom
Language eng
Subject 2403 Immunology
2723 Immunology and Allergy
Formatted abstract
Background: Chronic noninfectious, nonallergic rhinitis (NINAR) is a complex syndrome with a principally unknown pathophysiology. New technology has made it possible to examine differentially expressed genes and according to network theory, genes connected by their function that might have key roles in the disease.

Methods: Connectivity analysis was used to identify NINAR key genes. mRNA was extracted from nasal biopsies from 12 NINAR patients and 12 healthy volunteers. Microarrays were performed using Affymetrix chips with 54 613 genes. Data were analysed with the Ingenuity Pathway System for organization of genes into annotated biological functions and, thereafter, linking genes into networks due to their connectivity. The regulation of key genes was confirmed with reverse transcription-polymerase chain reaction (RT-PCR).

Results: In all, 43 genes were differentially expressed. The functional analysis showed that these genes were primarily involved in cellular movement, haematological system development and immune response. Merging these functions, 10 genes were found to be shared. Network analysis generated three networks and two of these 'shared genes' in key positions, c-fos and cell division cycle 42 (Cdc42). These genes were upregulated in both the array and the RT-PCR analysis.

Conclusion: Ten genes were found to be of pathophysiological interest for NINAR and of these, c-fos and Cdc42 seemed to be of specific interest due to their ability to interact with other genes of interest within this context. Although the role of c-fos and Cdc42 in upper airway inflammation remains unknown, they might be used as potential disease markers.
Keyword c-fos
Cell division cycle 42
Connectivity analysis
Ingenuity Pathway System
Microarray
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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